Affinity Capture-MS

An interaction is inferred when a bait protein is affinity captured from cell extracts by either polyclonal antibody or epitope tag and the associated interaction partner is identified by mass spectrometric methods.

Publication

Proteomic profiling and functional characterization of post-translational modifications of the fission yeast RNA exosome.

Telekawa C, Boisvert FM, Bachand F

The RNA exosome is a conserved multi-subunit complex essential for processing and degradation of several types of RNAs. Although many of the functions of the RNA exosome are well established, whether the activity of this complex is regulated remains unclear. Here we performed a proteomic analysis of the RNA exosome complex purified from Schizosaccharomyces pombe and identified 39 post-translational modifications ... [more]

Nucleic Acids Res. Dec. 30, 2017; 46(21);11169-11183 [Pubmed: 30321377]

Throughput

  • High Throughput

Curated By

  • BioGRID