An interaction is inferred when a bait protein is affinity captured from cell extracts by either polyclonal antibody or epitope tag and the associated interaction partner identified by Western blot with a specific polyclonal antibody or second epitope tag. This category is also used if an interacting protein is visualized directly by dye stain or radioactivity. Note that this differs from any co-purification experiment involving affinity capture in that the co-purification experiment involves at least one extra purification step to get rid of potential contaminating proteins.

Publication

Assembly of retinal rod or cone Na(+)/Ca(2+)-K(+) exchanger oligomers with cGMP-gated channel subunits as probed with heterologously expressed cDNAs.

Kang K, Bauer PJ, Kinjo TG, Szerencsei RT, Boenigk W, Winkfein RJ, Schnetkamp PP

Proper control of intracellular free Ca(2+) is thought to involve subsets of proteins that co-localize to mediate coordinated Ca(2+) entry and Ca(2+) extrusion. The outer segments of vertebrate rod and cone photoreceptors present one example: Ca(2+) influx is exclusively mediated via cGMP-gated channels (CNG), whereas the Na(+)/Ca(2+)-K(+) exchanger (NCKX) is the only Ca(2+) extrusion protein present. In situ, a rod ... [more]

Biochemistry Apr. 22, 2003; 42(15);4593-600 [Pubmed: 12693957]

Throughput

Low Throughput

Curated By

BioGRID

Interaction Summary

SLC24A1

Gene Ontology Biological Process

Gene Ontology Molecular Function

calcium, potassium:sodium antiporter activity [NAS]protein binding [IPI]

Gene Ontology Cellular Component