An interaction is inferred from the biochemical effect of one protein upon another, for example, GTP-GDP exchange activity or phosphorylation of a substrate by a kinase. The bait protein executes the activity on the substrate hit protein. A Modification value is recorded for interactions of this type with the possible values Phosphorylation, Ubiquitination, Sumoylation, Dephosphorylation, Methylation, Prenylation, Acetylation, Deubiquitination, Proteolytic Processing, Glucosylation, Nedd(Rub1)ylation, Deacetylation, No Modification, Demethylation.

Publication

Identification of sumoylation sites in CCDC6, the first identified RET partner gene in papillary thyroid carcinoma, uncovers a mode of regulating CCDC6 function on CREB1 transcriptional activity.

Luise C, Merolla F, Leone V, Paladino S, Sarnataro D, Fusco A, Celetti A

CCDC6 was originally identified in chimeric genes as caused by chromosomal translocation involving the RET protooncogene in some thyroid tumors. Recognised as a 65 kDa pro-apoptotic phosphoprotein, CCDC6 has been enrolled as an ATM substrate that contribute to protect genome integrity by modulating PP4c activity in response to genotoxic stress. Recently, CCDC6 has been identified as a repressor of CREB1-dependent ... [more]

PLoS ONE Nov. 13, 2012; 7(11);e49298 [Pubmed: 23145146]

Throughput

Low Throughput

Additional Notes

in vitro sumoylation using UBA2 and SAE1 as E1, Ubc9 as E2, either SUMO1 or, in a separate experiment, SUMO2 as modifiers, and CCDC6 as substrate

Curated By

BioGRID

Interaction Summary

UBE2I

Gene Ontology Biological Process

Gene Ontology Molecular Function

RING-like zinc finger domain binding [IPI]SUMO transferase activity [IDA]enzyme binding [IPI]poly(A) RNA binding [IDA]protein binding [IPI]transcription factor binding [IPI]ubiquitin protein ligase activity [IBA]ubiquitin protein ligase binding [IBA]ubiquitin-protein transferase activity [TAS]

Gene Ontology Cellular Component

CCDC6