An interaction is inferred from the biochemical effect of one protein upon another, for example, GTP-GDP exchange activity or phosphorylation of a substrate by a kinase. The bait protein executes the activity on the substrate hit protein. A Modification value is recorded for interactions of this type with the possible values Phosphorylation, Ubiquitination, Sumoylation, Dephosphorylation, Methylation, Prenylation, Acetylation, Deubiquitination, Proteolytic Processing, Glucosylation, Nedd(Rub1)ylation, Deacetylation, No Modification, Demethylation.

Publication

Local generation of fumarate promotes DNA repair through inhibition of histone H3 demethylation.

Jiang Y, Qian X, Shen J, Wang Y, Li X, Liu R, Xia Y, Chen Q, Peng G, Lin SY, Lu Z

Histone methylation regulates DNA repair. However, the mechanisms that underlie the regulation of histone methylation during this repair remain to be further defined. Here, we show that exposure to ionizing radiation induces DNA-PK-dependent phosphorylation of nuclear fumarase at ThrÂ 236, which leads to an interaction between fumarase and the histone variant H2A.Z at DNA double-strand break (DSB) regions. Locally generated fumarate ... [more]

Nat. Cell Biol. Sep. 01, 2015; 17(9);1158-68 [Pubmed: 26237645]

Throughput

Low Throughput

Additional Notes

in vitro phosphorylation using DNA-PKcs as kinase catalytic subunit, Ku70 and Ku80 as kinase accessory subunits with FH/fumarase as the substrate

Curated By

BioGRID

Interaction Summary

PRKDC

Gene Ontology Biological Process

Gene Ontology Molecular Function

DNA-dependent protein kinase activity [IDA]poly(A) RNA binding [IDA]protein binding [IPI]protein kinase activity [TAS]protein serine/threonine kinase activity [IDA]transcription factor binding [IPI]

Gene Ontology Cellular Component

FH