BAIT
TREM2
TREM-2, Trem2a, Trem2b, Trem2c, RP1-238O23.2
triggering receptor expressed on myeloid cells 2
GO Process (11)
GO Function (2)
GO Component (3)
Gene Ontology Biological Process
- axon guidance [TAS]
- dendritic cell differentiation [IDA]
- humoral immune response [TAS]
- innate immune response [TAS]
- positive regulation of C-C chemokine receptor CCR7 signaling pathway [IDA]
- positive regulation of CD40 signaling pathway [IDA]
- positive regulation of ERK1 and ERK2 cascade [IDA]
- positive regulation of antigen processing and presentation of peptide antigen via MHC class II [IDA]
- positive regulation of calcium-mediated signaling [IDA]
- positive regulation of peptidyl-tyrosine phosphorylation [IDA]
- positive regulation of protein localization to plasma membrane [IDA]
Gene Ontology Molecular Function
Gene Ontology Cellular Component
Homo sapiens
PREY
APOE
AD2, APO-E, LDLCQ5, LPG
apolipoprotein E
GO Process (68)
GO Function (14)
GO Component (19)
Gene Ontology Biological Process
- G-protein coupled receptor signaling pathway [IDA]
- N-methyl-D-aspartate receptor clustering [IDA]
- alpha-amino-3-hydroxy-5-methyl-4-isoxazole propionate selective glutamate receptor clustering [IDA]
- cGMP-mediated signaling [IDA]
- cholesterol efflux [IDA]
- cholesterol homeostasis [IDA]
- cholesterol metabolic process [IDA, IMP]
- chylomicron remnant clearance [IMP]
- cytoskeleton organization [TAS]
- fatty acid homeostasis [IDA]
- high-density lipoprotein particle assembly [IDA]
- high-density lipoprotein particle clearance [IDA]
- high-density lipoprotein particle remodeling [IGI]
- intracellular transport [TAS]
- lipoprotein metabolic process [TAS]
- long-chain fatty acid transport [IDA]
- negative regulation of MAP kinase activity [IDA]
- negative regulation of beta-amyloid formation [IDA]
- negative regulation of blood coagulation [IDA]
- negative regulation of blood vessel endothelial cell migration [IDA]
- negative regulation of cholesterol biosynthetic process [IDA]
- negative regulation of cholesterol efflux [IDA]
- negative regulation of dendritic spine development [IDA]
- negative regulation of dendritic spine maintenance [IDA]
- negative regulation of endothelial cell proliferation [IDA]
- negative regulation of inflammatory response [IC]
- negative regulation of lipid biosynthetic process [IDA]
- negative regulation of lipid transport across blood brain barrier [IDA]
- negative regulation of neuron death [IDA]
- negative regulation of phospholipid efflux [IDA]
- negative regulation of platelet activation [IDA]
- negative regulation of postsynaptic membrane organization [IDA]
- negative regulation of presynaptic membrane organization [IDA]
- nitric oxide mediated signal transduction [IDA]
- phospholipid efflux [IDA]
- phototransduction, visible light [TAS]
- positive regulation of beta-amyloid formation [IDA]
- positive regulation of cGMP biosynthetic process [IDA]
- positive regulation of cholesterol efflux [IDA, IGI]
- positive regulation of cholesterol esterification [IDA]
- positive regulation of dendritic spine development [IDA]
- positive regulation of dendritic spine maintenance [IDA]
- positive regulation of lipid biosynthetic process [IDA]
- positive regulation of lipid transport across blood brain barrier [IDA]
- positive regulation of low-density lipoprotein particle receptor catabolic process [IDA]
- positive regulation of membrane protein ectodomain proteolysis [IDA]
- positive regulation of neurofibrillary tangle assembly [IDA]
- positive regulation of neuron death [IDA]
- positive regulation of nitric-oxide synthase activity [IDA]
- positive regulation of phospholipid efflux [IDA]
- positive regulation of postsynaptic membrane organization [IDA]
- positive regulation of presynaptic membrane organization [IDA]
- protein import [IDA]
- receptor-mediated endocytosis [IDA]
- regulation of Cdc42 protein signal transduction [IDA]
- regulation of axon extension [TAS]
- regulation of beta-amyloid clearance [IDA]
- regulation of neuron death [IDA]
- regulation of neuronal synaptic plasticity [TAS]
- regulation of tau-protein kinase activity [IDA]
- response to reactive oxygen species [NAS]
- retinoid metabolic process [TAS]
- reverse cholesterol transport [IDA]
- small molecule metabolic process [TAS]
- synaptic transmission, cholinergic [TAS]
- triglyceride metabolic process [IDA, IMP]
- very-low-density lipoprotein particle clearance [IDA, IMP]
- very-low-density lipoprotein particle remodeling [IDA, IGI]
Gene Ontology Molecular Function- antioxidant activity [IDA]
- beta-amyloid binding [IDA]
- heparin binding [IDA]
- identical protein binding [IDA]
- lipid binding [IDA]
- lipid transporter activity [IDA]
- low-density lipoprotein particle receptor binding [IDA, IPI]
- metal chelating activity [IDA]
- phosphatidylcholine-sterol O-acyltransferase activator activity [IDA]
- phospholipid binding [IDA]
- protein binding [IPI]
- protein homodimerization activity [IPI]
- tau protein binding [IPI]
- very-low-density lipoprotein particle receptor binding [IDA, IPI]
- antioxidant activity [IDA]
- beta-amyloid binding [IDA]
- heparin binding [IDA]
- identical protein binding [IDA]
- lipid binding [IDA]
- lipid transporter activity [IDA]
- low-density lipoprotein particle receptor binding [IDA, IPI]
- metal chelating activity [IDA]
- phosphatidylcholine-sterol O-acyltransferase activator activity [IDA]
- phospholipid binding [IDA]
- protein binding [IPI]
- protein homodimerization activity [IPI]
- tau protein binding [IPI]
- very-low-density lipoprotein particle receptor binding [IDA, IPI]
Gene Ontology Cellular Component
- blood microparticle [IDA]
- chylomicron [IDA]
- cytoplasm [NAS, TAS]
- dendrite [NAS]
- early endosome [TAS]
- endocytic vesicle lumen [TAS]
- extracellular matrix [IDA]
- extracellular region [TAS]
- extracellular space [IDA]
- extracellular vesicular exosome [IDA]
- high-density lipoprotein particle [IDA]
- intermediate-density lipoprotein particle [IDA]
- low-density lipoprotein particle [IDA]
- membrane [IDA]
- neuronal cell body [NAS]
- nucleus [IDA]
- plasma membrane [TAS]
- very-low-density lipoprotein particle [IDA]
- vesicle [IDA]
Homo sapiens
Reconstituted Complex
An interaction is inferred between proteins in vitro. This can include proteins in recombinant form or proteins isolated directly from cells with recombinant or purified bait. For example, GST pull-down assays where a GST-tagged protein is first isolated and then used to fish interactors from cell lysates are considered reconstituted complexes (e.g. PUBMED: 14657240, Fig. 4A or PUBMED: 14761940, Fig. 5). This can also include gel-shifts, surface plasmon resonance, isothermal titration calorimetry (ITC) and bio-layer interferometry (BLI) experiments. The bait-hit directionality may not be clear for 2 interacting proteins. In these cases the directionality is up to the discretion of the curator.
Publication
TREM2 Binds to Apolipoproteins, Including APOE and CLU/APOJ, and Thereby Facilitates Uptake of Amyloid-Beta by Microglia.
Genetic variants of TREM2, a protein expressed selectively by microglia in the brain, are associated with Alzheimer's disease (AD). Starting from an unbiased protein microarray screen, we identified a set of lipoprotein particles (including LDL) and apolipoproteins (including CLU/APOJ and APOE) as ligands of TREM2. Binding of these ligands by TREM2 was abolished or reduced by disease-associated mutations. Overexpression of wild-type ... [more]
Neuron Dec. 20, 2015; 91(2);328-40 [Pubmed: 27477018]
Throughput
- Low Throughput
Additional Notes
- assayed using BLI (biolayer interferometry); some interactions required prior lipidation of the hit protein
Curated By
- BioGRID