FAF1
Gene Ontology Biological Process
- cell death [IMP]
- cytoplasmic sequestering of NF-kappaB [IMP]
- positive regulation of apoptotic process [NAS]
- positive regulation of protein complex assembly [IMP]
- proteasome-mediated ubiquitin-dependent protein catabolic process [NAS]
- regulation of protein catabolic process [IMP]
- regulation of protein kinase activity [NAS]
Gene Ontology Molecular Function
Gene Ontology Cellular Component
VAPB
Gene Ontology Biological Process
- COPII-coated vesicle budding [IMP]
- ER to Golgi vesicle-mediated transport [IMP]
- activation of signaling protein activity involved in unfolded protein response [IDA]
- cellular calcium ion homeostasis [IMP]
- endoplasmic reticulum organization [IMP]
- endoplasmic reticulum unfolded protein response [IMP]
- modulation by virus of host morphology or physiology [IDA]
- negative regulation by host of viral genome replication [IDA]
- negative regulation by host of viral release from host cell [IDA]
- negative regulation by virus of viral protein levels in host cell [IDA]
- positive regulation by host of viral genome replication [IDA]
- positive regulation by host of viral release from host cell [IDA]
- positive regulation of viral genome replication [IMP]
- small molecule metabolic process [TAS]
- sphingolipid biosynthetic process [TAS]
- sphingolipid metabolic process [TAS]
Gene Ontology Molecular Function
Gene Ontology Cellular Component
Affinity Capture-MS
An interaction is inferred when a bait protein is affinity captured from cell extracts by either polyclonal antibody or epitope tag and the associated interaction partner is identified by mass spectrometric methods.
Publication
FAF1 phosphorylation by AKT accumulates TGF-β type II receptor and drives breast cancer metastasis.
TGF-β is pro-metastatic for the late-stage breast cancer cells. Despite recent progress, the regulation of TGF-β type II receptor remains uncertain. Here we report that FAF1 destabilizes TβRII on the cell surface by recruiting the VCP/E3 ligase complex, thereby limiting excessive TGF-β response. Importantly, activated AKT directly phosphorylates FAF1 at Ser 582, which disrupts the FAF1-VCP complex and reduces FAF1 ... [more]
Throughput
- High Throughput
Related interactions
| Interaction | Experimental Evidence Code | Dataset | Throughput | Score | Curated By | Notes |
|---|---|---|---|---|---|---|
| FAF1 VAPB | Affinity Capture-MS Affinity Capture-MS An interaction is inferred when a bait protein is affinity captured from cell extracts by either polyclonal antibody or epitope tag and the associated interaction partner is identified by mass spectrometric methods. | High | - | BioGRID | - | |
| VAPB FAF1 | Affinity Capture-MS Affinity Capture-MS An interaction is inferred when a bait protein is affinity captured from cell extracts by either polyclonal antibody or epitope tag and the associated interaction partner is identified by mass spectrometric methods. | High | 0.3701 | BioGRID | 3218703 | |
| FAF1 VAPB | Affinity Capture-MS Affinity Capture-MS An interaction is inferred when a bait protein is affinity captured from cell extracts by either polyclonal antibody or epitope tag and the associated interaction partner is identified by mass spectrometric methods. | High | - | BioGRID | - | |
| FAF1 VAPB | Affinity Capture-Western Affinity Capture-Western An interaction is inferred when a bait protein is affinity captured from cell extracts by either polyclonal antibody or epitope tag and the associated interaction partner identified by Western blot with a specific polyclonal antibody or second epitope tag. This category is also used if an interacting protein is visualized directly by dye stain or radioactivity. Note that this differs from any co-purification experiment involving affinity capture in that the co-purification experiment involves at least one extra purification step to get rid of potential contaminating proteins. | Low | - | BioGRID | - | |
| VAPB FAF1 | Affinity Capture-Western Affinity Capture-Western An interaction is inferred when a bait protein is affinity captured from cell extracts by either polyclonal antibody or epitope tag and the associated interaction partner identified by Western blot with a specific polyclonal antibody or second epitope tag. This category is also used if an interacting protein is visualized directly by dye stain or radioactivity. Note that this differs from any co-purification experiment involving affinity capture in that the co-purification experiment involves at least one extra purification step to get rid of potential contaminating proteins. | Low | - | BioGRID | - | |
| FAF1 VAPB | Reconstituted Complex Reconstituted Complex An interaction is inferred between proteins in vitro. This can include proteins in recombinant form or proteins isolated directly from cells with recombinant or purified bait. For example, GST pull-down assays where a GST-tagged protein is first isolated and then used to fish interactors from cell lysates are considered reconstituted complexes (e.g. PUBMED: 14657240, Fig. 4A or PUBMED: 14761940, Fig. 5). This can also include gel-shifts, surface plasmon resonance, isothermal titration calorimetry (ITC) and bio-layer interferometry (BLI) experiments. The bait-hit directionality may not be clear for 2 interacting proteins. In these cases the directionality is up to the discretion of the curator. | Low | - | BioGRID | - |
Curated By
- BioGRID