Affinity Capture-MS

An interaction is inferred when a bait protein is affinity captured from cell extracts by either polyclonal antibody or epitope tag and the associated interaction partner is identified by mass spectrometric methods.

Publication

FAF1 phosphorylation by AKT accumulates TGF-β type II receptor and drives breast cancer metastasis.

Xie F, Jin K, Shao L, Fan Y, Tu Y, Li Y, Yang B, van Dam H, Ten Dijke P, Weng H, Dooley S, Wang S, Jia J, Jin J, Zhou F, Zhang L

TGF-β is pro-metastatic for the late-stage breast cancer cells. Despite recent progress, the regulation of TGF-β type II receptor remains uncertain. Here we report that FAF1 destabilizes TβRII on the cell surface by recruiting the VCP/E3 ligase complex, thereby limiting excessive TGF-β response. Importantly, activated AKT directly phosphorylates FAF1 at Ser 582, which disrupts the FAF1-VCP complex and reduces FAF1 ... [more]

Nat Commun Dec. 26, 2016; 8();15021 [Pubmed: 28443643]

Throughput

  • High Throughput

Curated By

  • BioGRID