BAIT

VPS4

CSC1, DID6, END13, GRD13, VPL4, VPT10, AAA family ATPase VPS4, L000002956, YPR173C

AAA-ATPase involved in multivesicular body (MVB) protein sorting; ATP-bound Vps4p localizes to endosomes and catalyzes ESCRT-III disassembly and membrane release; ATPase activity is activated by Vta1p; regulates cellular sterol metabolism

GO Process (6)

GO Function (3)

GO Component (4)

Gene Ontology Biological Process

intralumenal vesicle formation [IMP]

late endosome to vacuole transport [IMP, IPI]

late endosome to vacuole transport via multivesicular body sorting pathway [IMP]

protein homooligomerization [IDA]

protein retention in Golgi apparatus [IMP]

sterol metabolic process [IGI, IMP]

Gene Ontology Molecular Function

ATP binding [IDA, IMP]
ATPase activity [IDA, IMP]
protein homodimerization activity [IDA]

Gene Ontology Cellular Component

cytoplasm [IDA]

endoplasmic reticulum [IDA]

endosome [IDA]

membrane [IDA]

SGD Alliance of Genome Resources Entrez Gene RefSeq UniprotKB

Saccharomyces cerevisiae (S288c)

PREY

DSC2

YOL073C, YOL073C

Multi-transmembrane subunit of the DSC ubiquitin ligase complex; similar in sequence to rhomboid pseudoproteases Der1p and UBAC2 that function in ERAD; ortholog of fission yeast dsc2

UPS Project

GO Process (0)

GO Function (0)

GO Component (0)

SGD Alliance of Genome Resources Entrez Gene RefSeq UniprotKB

Saccharomyces cerevisiae (S288c)

Synthetic Growth Defect

A genetic interaction is inferred when mutations in separate genes, each of which alone causes a minimal phenotype, result in a significant growth defect under a given condition when combined in the same cell.

Publication

Endosome and Golgi-associated degradation (EGAD) of membrane proteins regulates sphingolipid metabolism.

Schmidt O, Weyer Y, Baumann V, Widerin MA, Eising S, Angelova M, Schleiffer A, Kremser L, Lindner H, Peter M, Froehlich F, Teis D

Cellular homeostasis requires the ubiquitin-dependent degradation of membrane proteins. This was assumed to be mediated exclusively either by endoplasmic reticulum-associated degradation (ERAD) or by endosomal sorting complexes required for transport (ESCRT)-dependent lysosomal degradation. We identified in Saccharomyces cerevisiae an additional pathway that selectively extracts membrane proteins at Golgi and endosomes for degradation by cytosolic proteasomes. One endogenous substrate of this ... [more]

EMBO J. Aug. 01, 2019; 38(15);e101433 [Pubmed: 31368600]

Throughput

Low Throughput

Ontology Terms

phenotype: vegetative growth (APO:0000106)

Related interactions

Interaction	Experimental Evidence Code	Dataset	Throughput	Score	Curated By	Notes
VPS4 DSC2	Negative Genetic Negative Genetic Mutations/deletions in separate genes, each of which alone causes a minimal phenotype, but when combined in the same cell results in a more severe fitness defect or lethality under a given condition. This term is reserved for high or low throughput studies with scores.	Costanzo M (2010)	High	-0.3047	BioGRID	422466
DSC2 VPS4	Negative Genetic Negative Genetic Mutations/deletions in separate genes, each of which alone causes a minimal phenotype, but when combined in the same cell results in a more severe fitness defect or lethality under a given condition. This term is reserved for high or low throughput studies with scores.	Costanzo M (2016)	High	-0.2536	BioGRID	2179670
VPS4 DSC2	Negative Genetic Negative Genetic Mutations/deletions in separate genes, each of which alone causes a minimal phenotype, but when combined in the same cell results in a more severe fitness defect or lethality under a given condition. This term is reserved for high or low throughput studies with scores.	Costanzo M (2016)	High	-0.2785	BioGRID	2196908

Curated By

BioGRID

Interaction Summary