Two-hybrid

Bait protein expressed as a DNA binding domain (DBD) fusion and prey expressed as a transcriptional activation domain (TAD) fusion and interaction measured by reporter gene activation.

Publication

PIR1, a novel phosphatase that exhibits high affinity to RNA . ribonucleoprotein complexes.

Yuan Y, Li DM, Sun H

Protein tyrosine phosphatases are involved in the regulation of important cellular processes such as signal transduction, cell cycle progression, and tumor suppression. Here we report the cloning and characterization of PIR1, a novel member in the dual-specificity phosphatase subfamily of the protein tyrosine phosphatases. PIR1 also contains two stretches of arginine-rich sequences. We have shown that the recombinant PIR1 protein ... [more]

J. Biol. Chem. Aug. 07, 1998; 273(32);20347-53 [Pubmed: 9685386]

Throughput

  • Low Throughput

Curated By

  • BioGRID