BAIT

FBLIM1

2410043F08Rik, Cal, Fblp1, Gt10, migfilin, migfilin(s), RP23-291H3.1
filamin binding LIM protein 1
GO Process (2)
GO Function (1)
GO Component (2)

Gene Ontology Molecular Function

Gene Ontology Cellular Component

Mus musculus
PREY

FLNA

ABP-280, Dilp2, F730004A14Rik, Fln1, GENA 379, filamin-1, RP23-436K3.1
filamin, alpha
GO Process (19)
GO Function (16)
GO Component (18)
Mus musculus

Affinity Capture-Western

An interaction is inferred when a bait protein is affinity captured from cell extracts by either polyclonal antibody or epitope tag and the associated interaction partner identified by Western blot with a specific polyclonal antibody or second epitope tag. This category is also used if an interacting protein is visualized directly by dye stain or radioactivity. Note that this differs from any co-purification experiment involving affinity capture in that the co-purification experiment involves at least one extra purification step to get rid of potential contaminating proteins.

Publication

Kindlin binds migfilin tandem LIM domains and regulates migfilin focal adhesion localization and recruitment dynamics.

Brahme NN, Harburger DS, Kemp-O'Brien K, Stewart R, Raghavan S, Parsons M, Calderwood DA

Focal adhesions (FAs), sites of tight adhesion to the extracellular matrix, are composed of clusters of transmembrane integrin adhesion receptors and intracellular proteins that link integrins to the actin cytoskeleton and signaling pathways. Two integrin-binding proteins present in FAs, kindlin-1 and kindlin-2, are important for integrin activation, FA formation, and signaling. Migfilin, originally identified in a yeast two-hybrid screen for ... [more]

J. Biol. Chem. Dec. 06, 2013; 288(49);35604-16 [Pubmed: 24165133]

Throughput

  • Low Throughput

Curated By

  • BioGRID