An interaction is inferred when a bait protein is affinity captured from cell extracts by either polyclonal antibody or epitope tag and the associated interaction partner identified by Western blot with a specific polyclonal antibody or second epitope tag. This category is also used if an interacting protein is visualized directly by dye stain or radioactivity. Note that this differs from any co-purification experiment involving affinity capture in that the co-purification experiment involves at least one extra purification step to get rid of potential contaminating proteins.

Publication

mRNA circularization by METTL3-eIF3h enhances translation and promotes oncogenesis.

Choe J, Lin S, Zhang W, Liu Q, Wang L, Ramirez-Moya J, Du P, Kim W, Tang S, Sliz P, Santisteban P, George RE, Richards WG, Wong KK, Locker N, Slack FJ, Gregory RI

N6-methyladenosine (m6A) modification of mRNA is emerging as an important regulator of gene expression that affects different developmental and biological processes, and altered m6A homeostasis is linked to cancer1-5. m6A modification is catalysed by METTL3 and enriched in the 3' untranslated region of a large subset of mRNAs at sites close to the stop codon5. METTL3 can promote translation but ... [more]

Nature Dec. 01, 2017; 561(7724);556-560 [Pubmed: 30232453]

Throughput

Low Throughput

Curated By

BioGRID

Interaction Summary

METTL3

Gene Ontology Biological Process

Gene Ontology Molecular Function

mRNA (2'-O-methyladenosine-N6-)-methyltransferase activity [IDA, IMP]

Gene Ontology Cellular Component

EIF3B