BAIT
BACH1
6230421P05Rik, AI323795
BTB and CNC homology 1
GO Process (7)
GO Function (7)
GO Component (4)
Gene Ontology Biological Process
- negative regulation of transcription from RNA polymerase II promoter [IDA, ISO]
- positive regulation of transcription from RNA polymerase II promoter [IDA]
- regulation of transcription from RNA polymerase II promoter in response to hypoxia [ISO]
- regulation of transcription involved in G1/S transition of mitotic cell cycle [ISO]
- regulation of transcription involved in G2/M transition of mitotic cell cycle [ISO]
- regulation of transcription, DNA-templated [IDA]
- transcription from RNA polymerase II promoter [ISO]
Gene Ontology Molecular Function- DNA binding [IDA]
- RNA polymerase II core promoter proximal region sequence-specific DNA binding transcription factor activity involved in negative regulation of transcription [ISO]
- RNA polymerase II distal enhancer sequence-specific DNA binding [IDA]
- RNA polymerase II distal enhancer sequence-specific DNA binding transcription factor activity involved in negative regulation of transcription [IDA]
- RNA polymerase II distal enhancer sequence-specific DNA binding transcription factor activity involved in positive regulation of transcription [IDA]
- protein binding [IPI]
- sequence-specific DNA binding transcription factor activity [IDA]
- DNA binding [IDA]
- RNA polymerase II core promoter proximal region sequence-specific DNA binding transcription factor activity involved in negative regulation of transcription [ISO]
- RNA polymerase II distal enhancer sequence-specific DNA binding [IDA]
- RNA polymerase II distal enhancer sequence-specific DNA binding transcription factor activity involved in negative regulation of transcription [IDA]
- RNA polymerase II distal enhancer sequence-specific DNA binding transcription factor activity involved in positive regulation of transcription [IDA]
- protein binding [IPI]
- sequence-specific DNA binding transcription factor activity [IDA]
Mus musculus
PREY
CKAP4
CLIMP-63, ERGIC-63, p63
cytoskeleton-associated protein 4
GO Process (0)
GO Function (1)
GO Component (3)
Gene Ontology Molecular Function
Gene Ontology Cellular Component
Homo sapiens
Affinity Capture-MS
An interaction is inferred when a bait protein is affinity captured from cell extracts by either polyclonal antibody or epitope tag and the associated interaction partner is identified by mass spectrometric methods.
Publication
Phosphorylation of BACH1 switches its function from transcription factor to mitotic chromosome regulator and promotes its interaction with HMMR.
The transcription repressor BACH1 performs mutually independent dual roles in transcription regulation and chromosome alignment during mitosis by supporting polar ejection force of mitotic spindle. We now found that the mitotic spindles became oblique relative to the adhesion surface following endogenous BACH1 depletion in HeLa cells. This spindle orientation rearrangement was rescued by re-expression of BACH1 depending on its interactions ... [more]
Biochem. J. Dec. 15, 2017; 475(5);981-1002 [Pubmed: 29459360]
Throughput
- High Throughput
Additional Notes
- the hit proteins are those human proteins from asynchronous (but not nocodazole-arrested) HeLa cells that were identified by MS (mass spectrometry) as interacting with mouse Bach1
Curated By
- BioGRID