GPR37
Gene Ontology Biological Process
Gene Ontology Molecular Function
Gene Ontology Cellular Component
GABARAPL2
Gene Ontology Biological Process
- autophagic vacuole assembly [IBA]
- autophagy [NAS]
- cellular response to nitrogen starvation [IBA]
- intra-Golgi vesicle-mediated transport [ISS]
- membrane fusion [IBA]
- mitochondrion degradation [IBA]
- negative regulation of proteasomal protein catabolic process [IMP]
- nucleophagy [IBA]
- positive regulation of ATPase activity [ISS]
Gene Ontology Molecular Function
Gene Ontology Cellular Component
Reconstituted Complex
An interaction is inferred between proteins in vitro. This can include proteins in recombinant form or proteins isolated directly from cells with recombinant or purified bait. For example, GST pull-down assays where a GST-tagged protein is first isolated and then used to fish interactors from cell lysates are considered reconstituted complexes (e.g. PUBMED: 14657240, Fig. 4A or PUBMED: 14761940, Fig. 5). This can also include gel-shifts, surface plasmon resonance, isothermal titration calorimetry (ITC) and bio-layer interferometry (BLI) experiments. The bait-hit directionality may not be clear for 2 interacting proteins. In these cases the directionality is up to the discretion of the curator.
Publication
A novel modelling mechanism of PAEL receptor and GABARAPL2 interaction involved in Parkinson's disease.
Parkin associated endothelin like receptor (PAELR) is G-protein coupled and ubiquitinated by parkin, promoting its degradation. In autosomal recessive Parkinson's disease, mutations in parkin lead to PAELR aggregation in the endoplasmic reticulum (ER), ER stress, neurotoxicity and cell death. We have identified previously that the protein kinase C interacting protein (PICK1) interacts with and regulates the expression and cell toxicity ... [more]
Throughput
- Low Throughput
Additional Notes
- assayed using pull-down experiments in which GST (glutathione S-transferase)-PAELR bound to GABARAPL2 purified from HeLa cells
Related interactions
| Interaction | Experimental Evidence Code | Dataset | Throughput | Score | Curated By | Notes |
|---|---|---|---|---|---|---|
| GPR37 GABARAPL2 | Two-hybrid Two-hybrid Bait protein expressed as a DNA binding domain (DBD) fusion and prey expressed as a transcriptional activation domain (TAD) fusion and interaction measured by reporter gene activation. | Low | - | BioGRID | - |
Curated By
- BioGRID