Bait protein expressed as a DNA binding domain (DBD) fusion and prey expressed as a transcriptional activation domain (TAD) fusion and interaction measured by reporter gene activation.

Publication

Analysis of zinc-fingers and homeoboxes (ZHX)-1-interacting proteins: molecular cloning and characterization of a member of the ZHX family, ZHX3.

Yamada K, Kawata H, Shou Z, Hirano S, Mizutani T, Yazawa T, Sekiguchi T, Yoshino M, Kajitani T, Miyamoto K

Human zinc-fingers and homeoboxes (ZHX) 1, a transcriptional repressor, was originally cloned as an interacting protein with the activation domain of the A subunit of nuclear factor-Y (NF-YA). As the first step in investigating the mechanism by which ZHX1 acts as a transcriptional repressor, we conducted a search of ZHX1-interacting proteins using a yeast two-hybrid system. Nuclear proteins such as ... [more]

Biochem. J. Jul. 01, 2003; 373(0);167-78 [Pubmed: 12659632]

Throughput

Low Throughput

Related interactions

Interaction	Experimental Evidence Code	Dataset	Throughput	Score	Curated By	Notes
ZHX1 ZHX1	Reconstituted Complex Reconstituted Complex An interaction is detected between purified proteins in vitro.	Hirano S (2002)	Low	-	BioGRID	-
ZHX1 ZHX1	Reconstituted Complex Reconstituted Complex An interaction is detected between purified proteins in vitro.	Yamada K (2002)	Low	-	BioGRID	-
ZHX1 ZHX1	Two-hybrid Two-hybrid Bait protein expressed as a DNA binding domain (DBD) fusion and prey expressed as a transcriptional activation domain (TAD) fusion and interaction measured by reporter gene activation.	Yamada K (2002)	Low	-	BioGRID	-

Curated By

BioGRID

Interaction Summary

ZHX1

Gene Ontology Biological Process

Gene Ontology Molecular Function

protein binding [IPI]protein heterodimerization activity [IDA]sequence-specific DNA binding transcription factor activity [IDA]transcription corepressor activity [IDA]

Gene Ontology Cellular Component