PREY

TRIB3

C20orf97, NIPK, SINK, SKIP3, TRB3, RP5-1103G7.7
tribbles pseudokinase 3
Homo sapiens

Reconstituted Complex

An interaction is inferred between proteins in vitro. This can include proteins in recombinant form or proteins isolated directly from cells with recombinant or purified bait. For example, GST pull-down assays where a GST-tagged protein is first isolated and then used to fish interactors from cell lysates are considered reconstituted complexes (e.g. PUBMED: 14657240, Fig. 4A or PUBMED: 14761940, Fig. 5). This can also include gel-shifts, surface plasmon resonance, isothermal titration calorimetry (ITC) and bio-layer interferometry (BLI) experiments. The bait-hit directionality may not be clear for 2 interacting proteins. In these cases the directionality is up to the discretion of the curator.

Publication

Human Tribbles 3 protects nuclear DNA from cytidine deamination by APOBEC3A.

Aynaud MM, Suspene R, Vidalain PO, Mussil B, Guetard D, Tangy F, Wain-Hobson S, Vartanian JP

The human polydeoxynucleotide cytidine deaminases APOBEC3A, APOBEC3C, and APOBEC3H are capable of mutating viral DNA in the nucleus, whereas APOBEC3A alone efficiently edits nuclear DNA. Deamination is rapidly followed by excision of uracil residues and can lead to double-stranded breaks. It is not known to which protein networks these DNA mutators belong. Using a yeast two-hybrid screen, we identified the ... [more]

J. Biol. Chem. Nov. 09, 2012; 287(46);39182-92 [Pubmed: 22977230]

Throughput

  • Low Throughput

Curated By

  • BioGRID