BAIT
HLA-DMB
D6S221E, RING7, DAAP-27A1.4
major histocompatibility complex, class II, DM beta
GO Process (5)
GO Function (1)
GO Component (2)
Gene Ontology Biological Process
- MHC class II protein complex assembly [IMP]
- antigen processing and presentation of exogenous peptide antigen via MHC class II [IMP, TAS]
- peptide antigen assembly with MHC class II protein complex [IDA]
- positive regulation of T cell activation via T cell receptor contact with antigen bound to MHC molecule on antigen presenting cell [IMP]
- positive regulation of T cell proliferation [IMP]
Gene Ontology Molecular Function
Gene Ontology Cellular Component
Homo sapiens
PREY
PSEN2
AD3L, AD4, CMD1V, PS2, STM2
presenilin 2
GO Process (13)
GO Function (2)
GO Component (23)
Gene Ontology Biological Process
- Notch receptor processing [IBA, TAS]
- Notch signaling pathway [TAS]
- amyloid precursor protein catabolic process [IBA, TAS]
- apoptotic signaling pathway [TAS]
- beta-amyloid metabolic process [IBA]
- calcium ion transport [IBA]
- membrane protein ectodomain proteolysis [IDA]
- membrane protein intracellular domain proteolysis [TAS]
- negative regulation of apoptotic process [IBA]
- neurotrophin TRK receptor signaling pathway [TAS]
- positive regulation of apoptotic process [TAS]
- positive regulation of catalytic activity [IDA]
- protein processing [IDA]
Gene Ontology Molecular Function
Gene Ontology Cellular Component
- Golgi apparatus [IDA]
- Z disc [IBA]
- apical plasma membrane [IBA]
- axon [IBA]
- cell cortex [IBA]
- cell surface [IBA]
- centrosome [IDA]
- ciliary rootlet [IBA]
- dendritic shaft [IBA]
- endoplasmic reticulum [IDA]
- growth cone [IBA]
- integral component of plasma membrane [IDA]
- kinetochore [IDA]
- lysosomal membrane [IBA]
- membrane [IDA]
- membrane raft [IBA]
- mitochondrial inner membrane [IBA]
- neuromuscular junction [IBA]
- neuronal cell body [IBA]
- nuclear inner membrane [IDA]
- perinuclear region of cytoplasm [IBA]
- plasma membrane [TAS]
- protein complex [IDA]
Homo sapiens
FRET
An interaction is inferred when close proximity of interaction partners is detected by fluorescence resonance energy transfer between pairs of fluorophore-labeled molecules, such as occurs between CFP (donor) and YFP (acceptor) fusion proteins.
Publication
LuTHy: a double-readout bioluminescence-based two-hybrid technology for quantitative mapping of protein-protein interactions in mammalian cells.
Information on protein-protein interactions (PPIs) is of critical importance for studying complex biological systems and developing therapeutic strategies. Here, we present a double-readout bioluminescence-based two-hybrid technology, termed LuTHy, which provides two quantitative scores in one experimental procedure when testing binary interactions. PPIs are first monitored in cells by quantification of bioluminescence resonance energy transfer (BRET) and, following cell lysis, are ... [more]
Mol. Syst. Biol. Dec. 11, 2017; 14(7);e8071 [Pubmed: 29997244]
Throughput
- High Throughput
Additional Notes
- BRET
Curated By
- BioGRID