NTRK2
Gene Ontology Biological Process
- activation of adenylate cyclase activity [TAS]
- brain-derived neurotrophic factor receptor signaling pathway [IMP]
- central nervous system neuron development [ISS]
- cerebral cortex development [ISS]
- learning [ISS]
- negative regulation of neuron apoptotic process [ISS]
- neuron differentiation [ISS]
- neuron migration [ISS]
- neurotrophin TRK receptor signaling pathway [TAS]
- positive regulation of MAPK cascade [ISS]
- positive regulation of axonogenesis [ISS]
- positive regulation of cell proliferation [ISS]
- positive regulation of gene expression [ISS]
- positive regulation of neuron projection development [ISS]
- positive regulation of phosphatidylinositol 3-kinase signaling [ISS]
- protein autophosphorylation [ISS]
- regulation of Rac GTPase activity [ISS]
- transmembrane receptor protein tyrosine kinase signaling pathway [TAS]
Gene Ontology Molecular Function
Gene Ontology Cellular Component
NTF4
Gene Ontology Biological Process
- adult locomotory behavior [ISS]
- cell-cell signaling [IBA]
- epidermis development [ISS]
- ganglion mother cell fate determination [ISS]
- long-term memory [ISS]
- negative regulation of neuron apoptotic process [IBA]
- neuron projection morphogenesis [IBA]
- regulation of neuron differentiation [IBA]
- sensory organ boundary specification [ISS]
- transmembrane receptor protein tyrosine kinase signaling pathway [IBA]
Gene Ontology Molecular Function
Gene Ontology Cellular Component
FRET
An interaction is inferred when close proximity of interaction partners is detected by fluorescence resonance energy transfer between pairs of fluorophore-labeled molecules, such as occurs between CFP (donor) and YFP (acceptor) fusion proteins.
Publication
LuTHy: a double-readout bioluminescence-based two-hybrid technology for quantitative mapping of protein-protein interactions in mammalian cells.
Information on protein-protein interactions (PPIs) is of critical importance for studying complex biological systems and developing therapeutic strategies. Here, we present a double-readout bioluminescence-based two-hybrid technology, termed LuTHy, which provides two quantitative scores in one experimental procedure when testing binary interactions. PPIs are first monitored in cells by quantification of bioluminescence resonance energy transfer (BRET) and, following cell lysis, are ... [more]
Throughput
- High Throughput
Additional Notes
- BRET
Related interactions
| Interaction | Experimental Evidence Code | Dataset | Throughput | Score | Curated By | Notes |
|---|---|---|---|---|---|---|
| NTF4 NTRK2 | Co-crystal Structure Co-crystal Structure Interaction directly demonstrated at the atomic level by X-ray crystallography. Also used for NMR or Electron Microscopy (EM) structures. If there is no obvious bait-hit directionality to the interaction involving 3 or more proteins, then the co-crystallized proteins should be listed as a complex. | Low | - | BioGRID | - | |
| NTRK2 NTF4 | Co-localization Co-localization Interaction inferred from two proteins that co-localize in the cell by indirect immunofluorescence only when in addition, if one gene is deleted, the other protein becomes mis-localized. Also includes co-dependent association of proteins with promoter DNA in chromatin immunoprecipitation experiments. | High | - | BioGRID | 1504888 | |
| NTF4 NTRK2 | FRET FRET An interaction is inferred when close proximity of interaction partners is detected by fluorescence resonance energy transfer between pairs of fluorophore-labeled molecules, such as occurs between CFP (donor) and YFP (acceptor) fusion proteins. | High | - | BioGRID | 2598506 | |
| NTF4 NTRK2 | Reconstituted Complex Reconstituted Complex An interaction is inferred between proteins in vitro. This can include proteins in recombinant form or proteins isolated directly from cells with recombinant or purified bait. For example, GST pull-down assays where a GST-tagged protein is first isolated and then used to fish interactors from cell lysates are considered reconstituted complexes (e.g. PUBMED: 14657240, Fig. 4A or PUBMED: 14761940, Fig. 5). This can also include gel-shifts, surface plasmon resonance, isothermal titration calorimetry (ITC) and bio-layer interferometry (BLI) experiments. The bait-hit directionality may not be clear for 2 interacting proteins. In these cases the directionality is up to the discretion of the curator. | Low | - | BioGRID | - |
Curated By
- BioGRID