TTR
Gene Ontology Biological Process
Gene Ontology Molecular Function
Gene Ontology Cellular Component
RBP4
Gene Ontology Biological Process
- cardiac muscle tissue development [ISS]
- embryonic organ morphogenesis [ISS]
- embryonic retina morphogenesis in camera-type eye [ISS]
- embryonic skeletal system development [ISS]
- eye development [IMP, ISS]
- female genitalia morphogenesis [ISS]
- gluconeogenesis [IMP, ISS]
- glucose homeostasis [IDA]
- heart development [ISS]
- heart trabecula formation [ISS]
- lung development [ISS]
- maintenance of gastrointestinal epithelium [IDA]
- negative regulation of cardiac muscle cell proliferation [ISS]
- phototransduction, visible light [TAS]
- positive regulation of immunoglobulin secretion [ISS]
- positive regulation of insulin secretion [IMP]
- response to retinoic acid [IDA]
- retinoid metabolic process [TAS]
- retinol metabolic process [IMP]
- retinol transport [IC]
- urinary bladder development [ISS]
- uterus development [ISS]
- vagina development [ISS]
Gene Ontology Molecular Function
Gene Ontology Cellular Component
FRET
An interaction is inferred when close proximity of interaction partners is detected by fluorescence resonance energy transfer between pairs of fluorophore-labeled molecules, such as occurs between CFP (donor) and YFP (acceptor) fusion proteins.
Publication
LuTHy: a double-readout bioluminescence-based two-hybrid technology for quantitative mapping of protein-protein interactions in mammalian cells.
Information on protein-protein interactions (PPIs) is of critical importance for studying complex biological systems and developing therapeutic strategies. Here, we present a double-readout bioluminescence-based two-hybrid technology, termed LuTHy, which provides two quantitative scores in one experimental procedure when testing binary interactions. PPIs are first monitored in cells by quantification of bioluminescence resonance energy transfer (BRET) and, following cell lysis, are ... [more]
Throughput
- High Throughput|Low Throughput
Additional Notes
- BRET
Related interactions
| Interaction | Experimental Evidence Code | Dataset | Throughput | Score | Curated By | Notes |
|---|---|---|---|---|---|---|
| TTR RBP4 | Affinity Capture-Luminescence Affinity Capture-Luminescence An interaction is inferred when a bait protein, tagged with luciferase, is enzymatically detected in immunoprecipitates of the prey protein as light emission. The prey protein is affinity captured from cell extracts by either polyclonal antibody or epitope tag. | High | - | BioGRID | - | |
| RBP4 TTR | Affinity Capture-Luminescence Affinity Capture-Luminescence An interaction is inferred when a bait protein, tagged with luciferase, is enzymatically detected in immunoprecipitates of the prey protein as light emission. The prey protein is affinity captured from cell extracts by either polyclonal antibody or epitope tag. | High | - | BioGRID | - | |
| TTR RBP4 | Co-purification Co-purification An interaction is inferred from the identification of two or more protein subunits in a purified protein complex, as obtained by classical biochemical fractionation or affinity purification and one or more additional fractionation steps. | Low | - | BioGRID | - |
Curated By
- BioGRID