BAIT
SLC18A2
SVAT, SVMT, VAT2, VMAT2
solute carrier family 18 (vesicular monoamine transporter), member 2
GO Process (8)
GO Function (1)
GO Component (5)
Gene Ontology Biological Process
- dopamine transport [TAS]
- monoamine transport [TAS]
- negative regulation of reactive oxygen species biosynthetic process [NAS]
- neurotransmitter loading into synaptic vesicle [TAS]
- neurotransmitter secretion [TAS]
- sequestering of neurotransmitter [NAS]
- synaptic transmission [TAS]
- transmembrane transport [TAS]
Gene Ontology Molecular Function
Gene Ontology Cellular Component
Homo sapiens
PREY
DNAJC5
CLN4, CLN4B, CSP, DNAJC5A, NCL
DnaJ (Hsp40) homolog, subfamily C, member 5
GO Process (5)
GO Function (0)
GO Component (6)
Gene Ontology Biological Process
Gene Ontology Cellular Component
Homo sapiens
FRET
An interaction is inferred when close proximity of interaction partners is detected by fluorescence resonance energy transfer between pairs of fluorophore-labeled molecules, such as occurs between CFP (donor) and YFP (acceptor) fusion proteins.
Publication
LuTHy: a double-readout bioluminescence-based two-hybrid technology for quantitative mapping of protein-protein interactions in mammalian cells.
Information on protein-protein interactions (PPIs) is of critical importance for studying complex biological systems and developing therapeutic strategies. Here, we present a double-readout bioluminescence-based two-hybrid technology, termed LuTHy, which provides two quantitative scores in one experimental procedure when testing binary interactions. PPIs are first monitored in cells by quantification of bioluminescence resonance energy transfer (BRET) and, following cell lysis, are ... [more]
Mol. Syst. Biol. Dec. 11, 2017; 14(7);e8071 [Pubmed: 29997244]
Throughput
- High Throughput
Additional Notes
- BRET
Curated By
- BioGRID