PXN
Gene Ontology Biological Process
- activation of MAPK activity [IDA]
- branching morphogenesis of an epithelial tube [IDA]
- cellular component movement [IMP]
- cytoskeleton organization [IMP]
- focal adhesion assembly [IDA]
- growth hormone receptor signaling pathway [ISO]
- integrin-mediated signaling pathway [IMP, TAS]
- lamellipodium assembly [IDA]
- peptidyl-tyrosine phosphorylation [IDA]
- positive regulation of protein kinase activity [IMP]
- regulation of cell shape [IMP]
- substrate adhesion-dependent cell spreading [IMP]
- transforming growth factor beta receptor signaling pathway [IDA, ISO]
Gene Ontology Molecular Function
Gene Ontology Cellular Component
GIT1
Gene Ontology Biological Process
Gene Ontology Molecular Function
Affinity Capture-Western
An interaction is inferred when a bait protein is affinity captured from cell extracts by either polyclonal antibody or epitope tag and the associated interaction partner identified by Western blot with a specific polyclonal antibody or second epitope tag. This category is also used if an interacting protein is visualized directly by dye stain or radioactivity. Note that this differs from any co-purification experiment involving affinity capture in that the co-purification experiment involves at least one extra purification step to get rid of potential contaminating proteins.
Publication
Specific dephosphorylation at tyr-554 of git1 by ptprz promotes its association with paxillin and hic-5.
G protein-coupled receptor kinase-interactor 1 (Git1) is involved in cell motility control by serving as an adaptor that links signaling proteins such as Pix and PAK to focal adhesion proteins. We previously demonstrated that Git1 was a multiply tyrosine-phosphorylated protein, its primary phosphorylation site was Tyr-554 in the vicinity of the focal adhesion targeting-homology (FAH) domain, and this site was ... [more]
Throughput
- Low Throughput
Related interactions
Interaction | Experimental Evidence Code | Dataset | Throughput | Score | Curated By | Notes |
---|---|---|---|---|---|---|
PXN GIT1 | Affinity Capture-Western Affinity Capture-Western An interaction is inferred when a bait protein is affinity captured from cell extracts by either polyclonal antibody or epitope tag and the associated interaction partner identified by Western blot with a specific polyclonal antibody or second epitope tag. This category is also used if an interacting protein is visualized directly by dye stain or radioactivity. Note that this differs from any co-purification experiment involving affinity capture in that the co-purification experiment involves at least one extra purification step to get rid of potential contaminating proteins. | Low | - | BioGRID | - |
Curated By
- BioGRID