BAIT

WIPI2

ATG18B, Atg21, WIPI-2, CGI-50
WD repeat domain, phosphoinositide interacting 2
Autophagy Project
Homo sapiens
PREY

CCT3

CCT-gamma, CCTG, PIG48, TCP-1-gamma, TRIC5, RP11-443G18.6
chaperonin containing TCP1, subunit 3 (gamma)
GO Process (3)
GO Function (2)
GO Component (6)

Gene Ontology Biological Process

Gene Ontology Molecular Function

Gene Ontology Cellular Component

Homo sapiens

Affinity Capture-MS

An interaction is inferred when a bait protein is affinity captured from cell extracts by either polyclonal antibody or epitope tag and the associated interaction partner is identified by mass spectrometric methods.

Publication

mTORC1-Regulated and HUWE1-Mediated WIPI2 Degradation Controls Autophagy Flux.

Wan W, You Z, Zhou L, Xu Y, Peng C, Zhou T, Yi C, Shi Y, Liu W

mTORC1, the major homeostatic sensor and responder, regulates cell catabolism mainly by targeting autophagy. Here, we show that mTORC1 directly controls autophagosome formation via phosphorylation of WIPI2, a critical protein in isolation membrane growth and elongation. mTORC1 phosphorylates Ser395 of WIPI2, directing WIPI2 to interact specifically with the E3 ubiquitin ligase HUWE1 for ubiquitination and proteasomal degradation. Physiological or pharmacological ... [more]

Mol. Cell Dec. 18, 2017; 72(2);303-315.e6 [Pubmed: 30340022]

Throughput

  • High Throughput

Curated By

  • BioGRID