BAIT
WIPI2
ATG18B, Atg21, WIPI-2, CGI-50
WD repeat domain, phosphoinositide interacting 2
GO Process (7)
GO Function (3)
GO Component (8)
Gene Ontology Biological Process
Gene Ontology Molecular Function
Gene Ontology Cellular Component
Homo sapiens
PREY
AP2S1
AP17, CLAPS2, FBH3, FBHOk, HHC3
adaptor-related protein complex 2, sigma 1 subunit
GO Process (11)
GO Function (3)
GO Component (5)
Gene Ontology Biological Process
- antigen processing and presentation of exogenous peptide antigen via MHC class II [TAS]
- axon guidance [TAS]
- clathrin coat assembly [TAS]
- clathrin-mediated endocytosis [TAS]
- epidermal growth factor receptor signaling pathway [TAS]
- negative regulation of epidermal growth factor receptor signaling pathway [TAS]
- neurotrophin TRK receptor signaling pathway [TAS]
- regulation of defense response to virus by virus [TAS]
- regulation of endocytosis [TAS]
- synaptic transmission [TAS]
- viral process [TAS]
Gene Ontology Molecular Function
Gene Ontology Cellular Component
Homo sapiens
Affinity Capture-MS
An interaction is inferred when a bait protein is affinity captured from cell extracts by either polyclonal antibody or epitope tag and the associated interaction partner is identified by mass spectrometric methods.
Publication
mTORC1-Regulated and HUWE1-Mediated WIPI2 Degradation Controls Autophagy Flux.
mTORC1, the major homeostatic sensor and responder, regulates cell catabolism mainly by targeting autophagy. Here, we show that mTORC1 directly controls autophagosome formation via phosphorylation of WIPI2, a critical protein in isolation membrane growth and elongation. mTORC1 phosphorylates Ser395 of WIPI2, directing WIPI2 to interact specifically with the E3 ubiquitin ligase HUWE1 for ubiquitination and proteasomal degradation. Physiological or pharmacological ... [more]
Mol. Cell Dec. 18, 2017; 72(2);303-315.e6 [Pubmed: 30340022]
Throughput
- High Throughput
Curated By
- BioGRID