PREY

NOXO1

P41NOX, P41NOXA, P41NOXB, P41NOXC, SH3PXD5, SNX28
NADPH oxidase organizer 1
Homo sapiens

Reconstituted Complex

An interaction is inferred between proteins in vitro. This can include proteins in recombinant form or proteins isolated directly from cells with recombinant or purified bait. For example, GST pull-down assays where a GST-tagged protein is first isolated and then used to fish interactors from cell lysates are considered reconstituted complexes (e.g. PUBMED: 14657240, Fig. 4A or PUBMED: 14761940, Fig. 5). This can also include gel-shifts, surface plasmon resonance, isothermal titration calorimetry (ITC) and bio-layer interferometry (BLI) experiments. The bait-hit directionality may not be clear for 2 interacting proteins. In these cases the directionality is up to the discretion of the curator.

Publication

Molecular interaction of NADPH oxidase 1 with betaPix and Nox Organizer 1.

Park HS, Park D, Bae YS

It is well established that growth-factor-induced reactive oxygen species (ROS) act as second messengers in cell signaling. We have previously reported that betaPix, a guanine nucleotide exchange factor for Rac, interacts with NADPH oxidase 1 (Nox1) leading to EGF-induced ROS generation. Here, we report the identification of the domains of Nox1 and betaPix responsible for the interaction between the two ... [more]

Biochem. Biophys. Res. Commun. Jan. 20, 2006; 339(3);985-90 [Pubmed: 16329988]

Throughput

  • Low Throughput

Curated By

  • BioGRID