BAIT

ORC3

LAT, LATHEO, ORC3L, RP3-486L4.1
origin recognition complex, subunit 3
GO Process (3)
GO Function (2)
GO Component (3)
Homo sapiens
PREY

MCM7

CDC47, MCM2, P1.1-MCM3, P1CDC47, P85MCM, PNAS146, PPP1R104
minichromosome maintenance complex component 7
Homo sapiens

Two-hybrid

Bait protein expressed as a DNA binding domain (DBD) fusion and prey expressed as a transcriptional activation domain (TAD) fusion and interaction measured by reporter gene activation.

Publication

Interaction and assembly of murine pre-replicative complex proteins in yeast and mouse cells.

Kneissl M, Puetter V, Szalay AA, Grummt F

Eukaryotic cells coordinate chromosome duplication by the assembly of protein complexes at origins of DNA replication by sequential binding of member proteins of the origin recognition complex (ORC), CDC6, and minichromosome maintenance (MCM) proteins. These pre-replicative complexes (pre-RCs) are activated by cyclin-dependent kinases and DBF4/CDC7 kinase. Here, we carried out a comprehensive yeast two-hybrid screen to establish sequential interactions between ... [more]

J. Mol. Biol. Mar. 14, 2003; 327(1);111-28 [Pubmed: 12614612]

Throughput

  • High Throughput

Related interactions

InteractionExperimental Evidence CodeDatasetThroughputScoreCurated ByNotes
ORC3 MCM7
Reconstituted Complex
Reconstituted Complex

An interaction is detected between purified proteins in vitro.

High-BioGRID
-
MCM7 ORC3
Two-hybrid
Two-hybrid

Bait protein expressed as a DNA binding domain (DBD) fusion and prey expressed as a transcriptional activation domain (TAD) fusion and interaction measured by reporter gene activation.

High-BioGRID
-

Curated By

  • BioGRID