CDC7
Gene Ontology Biological Process
- DNA replication [TAS]
- DNA replication initiation [IBA]
- G1/S transition of mitotic cell cycle [TAS]
- cell cycle phase transition [IMP]
- double-strand break repair via break-induced replication [IBA]
- mitotic cell cycle [TAS]
- peptidyl-serine phosphorylation [IBA]
- phagocytosis [IBA]
- positive regulation of G2/M transition of mitotic cell cycle [IMP]
- positive regulation of cell proliferation [IMP]
- positive regulation of nuclear cell cycle DNA replication [IMP]
- regulation of cell shape [IBA]
Gene Ontology Molecular Function
Gene Ontology Cellular Component
MCM4
Gene Ontology Biological Process
Gene Ontology Molecular Function
Gene Ontology Cellular Component
- MCM complex [IDA]
- membrane [IDA]
- nucleoplasm [IDA, TAS]
- nucleus [NAS]
Two-hybrid
Bait protein expressed as a DNA binding domain (DBD) fusion and prey expressed as a transcriptional activation domain (TAD) fusion and interaction measured by reporter gene activation.
Publication
Interaction and assembly of murine pre-replicative complex proteins in yeast and mouse cells.
Eukaryotic cells coordinate chromosome duplication by the assembly of protein complexes at origins of DNA replication by sequential binding of member proteins of the origin recognition complex (ORC), CDC6, and minichromosome maintenance (MCM) proteins. These pre-replicative complexes (pre-RCs) are activated by cyclin-dependent kinases and DBF4/CDC7 kinase. Here, we carried out a comprehensive yeast two-hybrid screen to establish sequential interactions between ... [more]
Throughput
- High Throughput
Related interactions
Interaction | Experimental Evidence Code | Dataset | Throughput | Score | Curated By | Notes |
---|---|---|---|---|---|---|
CDC7 MCM4 | Biochemical Activity Biochemical Activity An interaction is inferred from the biochemical effect of one protein upon another, for example, GTP-GDP exchange activity or phosphorylation of a substrate by a kinase. The bait protein executes the activity on the substrate hit protein. A Modification value is recorded for interactions of this type with the possible values Phosphorylation, Ubiquitination, Sumoylation, Dephosphorylation, Methylation, Prenylation, Acetylation, Deubiquitination, Proteolytic Processing, Glucosylation, Nedd(Rub1)ylation, Deacetylation, No Modification, Demethylation. | Low | - | BioGRID | 811793 | |
CDC7 MCM4 | Biochemical Activity Biochemical Activity An interaction is inferred from the biochemical effect of one protein upon another, for example, GTP-GDP exchange activity or phosphorylation of a substrate by a kinase. The bait protein executes the activity on the substrate hit protein. A Modification value is recorded for interactions of this type with the possible values Phosphorylation, Ubiquitination, Sumoylation, Dephosphorylation, Methylation, Prenylation, Acetylation, Deubiquitination, Proteolytic Processing, Glucosylation, Nedd(Rub1)ylation, Deacetylation, No Modification, Demethylation. | Low | - | BioGRID | 2533350 | |
MCM4 CDC7 | Two-hybrid Two-hybrid Bait protein expressed as a DNA binding domain (DBD) fusion and prey expressed as a transcriptional activation domain (TAD) fusion and interaction measured by reporter gene activation. | High | - | BioGRID | - |
Curated By
- BioGRID