SMAD7
Gene Ontology Biological Process
- BMP signaling pathway [TAS]
- adherens junction assembly [IMP]
- artery morphogenesis [ISS]
- cellular protein complex localization [IDA]
- cellular response to transforming growth factor beta stimulus [IMP]
- gene expression [TAS]
- negative regulation of BMP signaling pathway [IDA]
- negative regulation of cell migration [TAS]
- negative regulation of epithelial to mesenchymal transition [IC, TAS]
- negative regulation of pathway-restricted SMAD protein phosphorylation [IDA, TAS]
- negative regulation of peptidyl-serine phosphorylation [IDA]
- negative regulation of peptidyl-threonine phosphorylation [IDA]
- negative regulation of protein ubiquitination [IDA]
- negative regulation of sequence-specific DNA binding transcription factor activity [IDA]
- negative regulation of transcription by competitive promoter binding [IDA]
- negative regulation of transcription from RNA polymerase II promoter [IDA]
- negative regulation of transforming growth factor beta receptor signaling pathway [IDA, TAS]
- negative regulation of ubiquitin-protein transferase activity [IDA]
- pathway-restricted SMAD protein phosphorylation [ISS]
- positive regulation of cell-cell adhesion [IDA]
- positive regulation of proteasomal ubiquitin-dependent protein catabolic process [IDA]
- positive regulation of protein ubiquitination [IDA]
- positive regulation of transcription from RNA polymerase II promoter [TAS]
- protein stabilization [IDA]
- regulation of activin receptor signaling pathway [IDA]
- regulation of cardiac muscle contraction [ISS]
- regulation of transforming growth factor beta receptor signaling pathway [IC]
- regulation of ventricular cardiac muscle cell membrane depolarization [IC]
- response to laminar fluid shear stress [IEP]
- transcription initiation from RNA polymerase II promoter [TAS]
- transcription, DNA-templated [TAS]
- transforming growth factor beta receptor signaling pathway [TAS]
- ventricular cardiac muscle tissue morphogenesis [ISS]
- ventricular septum morphogenesis [ISS]
Gene Ontology Molecular Function
- I-SMAD binding [IPI]
- activin binding [IPI]
- beta-catenin binding [IPI]
- protein binding [IPI]
- transcription regulatory region DNA binding [IDA]
- transforming growth factor beta receptor, inhibitory cytoplasmic mediator activity [IDA]
- type I transforming growth factor beta receptor binding [IPI]
- ubiquitin protein ligase binding [IPI]
Gene Ontology Cellular Component
MAP2K6
Gene Ontology Biological Process
- DNA damage induced protein phosphorylation [TAS]
- MyD88-dependent toll-like receptor signaling pathway [TAS]
- MyD88-independent toll-like receptor signaling pathway [TAS]
- TRIF-dependent toll-like receptor signaling pathway [TAS]
- activation of MAPK activity [TAS]
- cell cycle arrest [TAS]
- innate immune response [TAS]
- muscle cell differentiation [TAS]
- nucleotide-binding domain, leucine rich repeat containing receptor signaling pathway [TAS]
- nucleotide-binding oligomerization domain containing signaling pathway [TAS]
- positive regulation of muscle cell differentiation [TAS]
- signal transduction [TAS]
- stress-activated MAPK cascade [TAS]
- toll-like receptor 10 signaling pathway [TAS]
- toll-like receptor 2 signaling pathway [TAS]
- toll-like receptor 3 signaling pathway [TAS]
- toll-like receptor 4 signaling pathway [TAS]
- toll-like receptor 5 signaling pathway [TAS]
- toll-like receptor 9 signaling pathway [TAS]
- toll-like receptor TLR1:TLR2 signaling pathway [TAS]
- toll-like receptor TLR6:TLR2 signaling pathway [TAS]
- toll-like receptor signaling pathway [TAS]
Gene Ontology Molecular Function
Gene Ontology Cellular Component
- cytosol [TAS]
- nucleoplasm [TAS]
Affinity Capture-Western
An interaction is inferred when a bait protein is affinity captured from cell extracts by either polyclonal antibody or epitope tag and the associated interaction partner identified by Western blot with a specific polyclonal antibody or second epitope tag. This category is also used if an interacting protein is visualized directly by dye stain or radioactivity. Note that this differs from any co-purification experiment involving affinity capture in that the co-purification experiment involves at least one extra purification step to get rid of potential contaminating proteins.
Publication
Transforming growth factor-beta1 (TGF-beta)-induced apoptosis of prostate cancer cells involves Smad7-dependent activation of p38 by TGF-beta-activated kinase 1 and mitogen-activated protein kinase kinase 3.
The inhibitory Smad7, a direct target gene for transforming growth factor-beta (TGF-beta), mediates TGF-beta1-induced apoptosis in several cell types. Herein, we report that apoptosis of human prostate cancer PC-3U cells induced by TGF-beta1 or Smad7 overexpression is caused by a specific activation of the p38 mitogen-activated protein kinase pathway in a TGF-beta-activated kinase 1 (TAK1)- and mitogen-activated protein kinase kinase ... [more]
Throughput
- Low Throughput
Curated By
- BioGRID