BAIT

CSM3

YMR048W
Replication fork associated factor; required for stable replication fork pausing; component of the DNA replication checkpoint pathway; required for accurate chromosome segregation during meiosis; forms nuclear foci upon DNA replication stress
Saccharomyces cerevisiae (S288c)
PREY

SLX4

YLR135W
Endonuclease involved in processing DNA; acts during recombination and repair; promotes template switching during break-induced replication (BIR), causing non-reciprocal translocations (NRTs); cleaves branched structures in a complex with Slx1p; involved interstrand cross-link repair and in Rad1p/Rad10p-dependent removal of 3'-nonhomologous tails during DSBR via single-strand annealing; relative distribution to nuclear foci increases upon DNA replication stress
Saccharomyces cerevisiae (S288c)

Negative Genetic

Mutations/deletions in separate genes, each of which alone causes a minimal phenotype, but when combined in the same cell results in a more severe fitness defect or lethality under a given condition. This term is reserved for high or low throughput studies with scores.

Publication

Mapping DNA damage-dependent genetic interactions in yeast via party mating and barcode fusion genetics.

Diaz-Mejia JJ, Celaj A, Mellor JC, Cote A, Balint A, Ho B, Bansal P, Shaeri F, Gebbia M, Weile J, Verby M, Karkhanina A, Zhang Y, Wong C, Rich J, Prendergast D, Gupta G, Oeztuerk S, Durocher D, Brown GW, Roth FP

Condition-dependent genetic interactions can reveal functional relationships between genes that are not evident under standard culture conditions. State-of-the-art yeast genetic interaction mapping, which relies on robotic manipulation of arrays of double-mutant strains, does not scale readily to multi-condition studies. Here, we describe barcode fusion genetics to map genetic interactions (BFG-GI), by which double-mutant strains generated via en masse "party" mating ... [more]

Mol. Syst. Biol. Dec. 28, 2017; 14(5);e7985 [Pubmed: 29807908]

Quantitative Score

  • -0.12273402 [Confidence Score]

Throughput

  • High Throughput

Ontology Terms

  • phenotype: vegetative growth (APO:0000106)

Additional Notes

  • Cultures grown in 4-NQO (CHEBI:16907)
  • Cultures grown in MMS ((CHEBI:25255))
  • Interactions determined by barcode fusion genetics to map genetic interactions (BFG-GI) using a ZGenetic Interaction Score (GIS)cutoff corresponding to FDR=0.01 and an additional effect?size cutoff (-0.075>GIS>0.075)

Related interactions

InteractionExperimental Evidence CodeDatasetThroughputScoreCurated ByNotes
SLX4 CSM3
Negative Genetic
Negative Genetic

Mutations/deletions in separate genes, each of which alone causes a minimal phenotype, but when combined in the same cell results in a more severe fitness defect or lethality under a given condition. This term is reserved for high or low throughput studies with scores.

High-BioGRID
938776
CSM3 SLX4
Negative Genetic
Negative Genetic

Mutations/deletions in separate genes, each of which alone causes a minimal phenotype, but when combined in the same cell results in a more severe fitness defect or lethality under a given condition. This term is reserved for high or low throughput studies with scores.

Low-0.0983BioGRID
560487

Curated By

  • BioGRID