BAIT

RTT101

CUL8, cullin RTT101, CULC, L000004737, YJL047C
Cullin subunit of a Roc1p-dependent E3 ubiquitin ligase complex; role in anaphase progression; implicated in Mms22-dependent DNA repair; involved with Mms1p in nonfunctional rRNA decay; modified by the ubiquitin-like protein, Rub1p
UPS Project
Saccharomyces cerevisiae (S288c)
PREY

SLX4

YLR135W
Endonuclease involved in processing DNA; acts during recombination and repair; promotes template switching during break-induced replication (BIR), causing non-reciprocal translocations (NRTs); cleaves branched structures in a complex with Slx1p; involved interstrand cross-link repair and in Rad1p/Rad10p-dependent removal of 3'-nonhomologous tails during DSBR via single-strand annealing; relative distribution to nuclear foci increases upon DNA replication stress
Saccharomyces cerevisiae (S288c)

Negative Genetic

Mutations/deletions in separate genes, each of which alone causes a minimal phenotype, but when combined in the same cell results in a more severe fitness defect or lethality under a given condition. This term is reserved for high or low throughput studies with scores.

Publication

Mapping DNA damage-dependent genetic interactions in yeast via party mating and barcode fusion genetics.

Diaz-Mejia JJ, Celaj A, Mellor JC, Cote A, Balint A, Ho B, Bansal P, Shaeri F, Gebbia M, Weile J, Verby M, Karkhanina A, Zhang Y, Wong C, Rich J, Prendergast D, Gupta G, Oeztuerk S, Durocher D, Brown GW, Roth FP

Condition-dependent genetic interactions can reveal functional relationships between genes that are not evident under standard culture conditions. State-of-the-art yeast genetic interaction mapping, which relies on robotic manipulation of arrays of double-mutant strains, does not scale readily to multi-condition studies. Here, we describe barcode fusion genetics to map genetic interactions (BFG-GI), by which double-mutant strains generated via en masse "party" mating ... [more]

Mol. Syst. Biol. Dec. 28, 2017; 14(5);e7985 [Pubmed: 29807908]

Quantitative Score

  • -0.32881106 [Confidence Score]

Throughput

  • High Throughput

Ontology Terms

  • phenotype: vegetative growth (APO:0000106)

Additional Notes

  • Cultures grown in MMS ((CHEBI:25255))
  • Interactions determined by barcode fusion genetics to map genetic interactions (BFG-GI) using a ZGenetic Interaction Score (GIS)cutoff corresponding to FDR=0.01 and an additional effect?size cutoff (-0.075>GIS>0.075)

Related interactions

InteractionExperimental Evidence CodeDatasetThroughputScoreCurated ByNotes
SLX4 RTT101
Negative Genetic
Negative Genetic

Mutations/deletions in separate genes, each of which alone causes a minimal phenotype, but when combined in the same cell results in a more severe fitness defect or lethality under a given condition. This term is reserved for high or low throughput studies with scores.

High-BioGRID
938798
RTT101 SLX4
Negative Genetic
Negative Genetic

Mutations/deletions in separate genes, each of which alone causes a minimal phenotype, but when combined in the same cell results in a more severe fitness defect or lethality under a given condition. This term is reserved for high or low throughput studies with scores.

Low-0.1069BioGRID
560495
SLX4 RTT101
Synthetic Rescue
Synthetic Rescue

A genetic interaction is inferred when mutations or deletions of one gene rescues the lethality or growth defect of a strain mutated or deleted for another gene.

Low-BioGRID
2388510

Curated By

  • BioGRID