BAIT

TNFRSF1A

CD120a, FPF, TNF-R, TNF-R-I, TNF-R1, TNF-R55, TNF-alphaR1, TNFAR, TNFR60, TNFRI, TNFRp55, TNFalpha-R1, Tnfr-2, Tnfr1, p55, p55-R
tumor necrosis factor receptor superfamily, member 1a
GO Process (23)
GO Function (5)
GO Component (13)
Mus musculus
PREY

CYBA

b558, nmf333, p22-phox, p22phox
cytochrome b-245, alpha polypeptide
Mus musculus

Affinity Capture-Western

An interaction is inferred when a bait protein is affinity captured from cell extracts by either polyclonal antibody or epitope tag and the associated interaction partner identified by Western blot with a specific polyclonal antibody or second epitope tag. This category is also used if an interacting protein is visualized directly by dye stain or radioactivity. Note that this differs from any co-purification experiment involving affinity capture in that the co-purification experiment involves at least one extra purification step to get rid of potential contaminating proteins.

Publication

Riboflavin kinase couples TNF receptor 1 to NADPH oxidase.

Yazdanpanah B, Wiegmann K, Tchikov V, Krut O, Pongratz C, Schramm M, Kleinridders A, Wunderlich T, Kashkar H, Utermoehlen O, Bruening JC, Schuetze S, Kroenke M

Reactive oxygen species (ROS) produced by NADPH oxidase function as defence and signalling molecules related to innate immunity and various cellular responses. The activation of NADPH oxidase in response to plasma membrane receptor activation depends on the phosphorylation of cytoplasmic oxidase subunits, their translocation to membranes and the assembly of all NADPH oxidase components. Tumour necrosis factor (TNF) is a ... [more]

Nature Aug. 27, 2009; 460(7259);1159-63 [Pubmed: 19641494]

Throughput

  • Low Throughput

Curated By

  • BioGRID