BAIT
TRPC6
FSGS2, TRP6
transient receptor potential cation channel, subfamily C, member 6
GO Process (9)
GO Function (2)
GO Component (1)
Gene Ontology Biological Process
- axon guidance [TAS]
- blood coagulation [TAS]
- calcium ion transmembrane transport [TAS]
- cation transport [TAS]
- ion transmembrane transport [TAS]
- platelet activation [TAS]
- positive regulation of calcium ion transport [IDA]
- positive regulation of ion transmembrane transporter activity [IDA]
- transmembrane transport [TAS]
Gene Ontology Molecular Function
Gene Ontology Cellular Component
Homo sapiens
PREY
RAB14
FBP, RAB-14, RP11-165P4.4
RAB14, member RAS oncogene family
GO Process (12)
GO Function (5)
GO Component (19)
Gene Ontology Biological Process
- GTP catabolic process [IDA]
- Golgi to endosome transport [ISS, TAS]
- Rab protein signal transduction [IBA]
- embryo development [ISS]
- endocytic recycling [IDA]
- fibroblast growth factor receptor signaling pathway [ISS]
- intracellular protein transport [IBA]
- intracellular transport [NAS]
- membrane organization [TAS]
- phagolysosome assembly involved in apoptotic cell clearance [IBA]
- regulation of protein localization [IDA]
- vesicle-mediated transport [NAS]
Gene Ontology Molecular Function
Gene Ontology Cellular Component
- Golgi apparatus [IBA]
- Golgi stack [ISS]
- cytoplasmic vesicle membrane [TAS]
- cytosol [ISS]
- early endosome [IBA, ISS]
- extracellular vesicular exosome [IDA]
- intracellular [IDA]
- intracellular membrane-bounded organelle [IDA]
- late endosome [ISS]
- lysosomal membrane [IDA]
- lysosome [ISS]
- nuclear outer membrane-endoplasmic reticulum membrane network [ISS]
- perinuclear region of cytoplasm [ISS]
- phagocytic vesicle [IDA]
- plasma membrane [ISS]
- primary cilium [IDA]
- recycling endosome [IDA]
- rough endoplasmic reticulum [ISS]
- trans-Golgi network transport vesicle [ISS]
Homo sapiens
Positive Genetic
Mutations/deletions in separate genes, each of which alone causes a minimal phenotype, but when combined in the same cell results in a less severe fitness defect than expected under a given condition. This term is reserved for high or low throughput studies with scores.
Publication
Transmembrane insertases and N-glycosylation critically determine synthesis, trafficking, and activity of the nonselective cation channel TRPC6.
Transient receptor potential cation channel subfamily C member 6 (TRPC6) is a widely expressed ion channel. Gain-of-function mutations in the human TRPC6 channel cause autosomal-dominant focal segmental glomerulosclerosis, but the molecular components involved in disease development remain unclear. Here, we found that overexpression of gain-of-function TRPC6 channel variants is cytotoxic in cultured cells. Exploiting this phenotype in a genome-wide CRISPR/Cas ... [more]
J. Biol. Chem. Aug. 23, 2019; 294(34);12655-12669 [Pubmed: 31266804]
Throughput
- High Throughput
Ontology Terms
- phenotype: growth abnormality (HP:0001507)
- phenotype: viability (PATO:0000169)
Additional Notes
- CRISPR GI screen
- Cell Line: TRex 293
- Experimental Setup: Positive Selection Screen
- GIST: A-phenotypic positive genetic interaction
- Hit genes conferred resistance to the cytotoxicity seen upon expression of a TRCP GOF mutant
- Library: Brunello Human Library
- Significance Threshold:NScore>3.4
Curated By
- BioGRID