BAIT

SETDB1

ESET, H3-K9-HMTase4, KG1T, KMT1E, TDRD21, RP11-316M1.1
SET domain, bifurcated 1
GO Process (0)
GO Function (1)
GO Component (5)

Gene Ontology Molecular Function

Gene Ontology Cellular Component

Homo sapiens
PREY

HIST2H3A

H3/n, H3/o
histone cluster 2, H3a
GO Process (1)
GO Function (1)
GO Component (4)

Gene Ontology Biological Process

Gene Ontology Molecular Function

Gene Ontology Cellular Component

Homo sapiens

Reconstituted Complex

An interaction is inferred between proteins in vitro. This can include proteins in recombinant form or proteins isolated directly from cells with recombinant or purified bait. For example, GST pull-down assays where a GST-tagged protein is first isolated and then used to fish interactors from cell lysates are considered reconstituted complexes (e.g. PUBMED: 14657240, Fig. 4A or PUBMED: 14761940, Fig. 5). This can also include gel-shifts, surface plasmon resonance, isothermal titration calorimetry (ITC) and bio-layer interferometry (BLI) experiments. The bait-hit directionality may not be clear for 2 interacting proteins. In these cases the directionality is up to the discretion of the curator.

Publication

SETDB1: a novel KAP-1-associated histone H3, lysine 9-specific methyltransferase that contributes to HP1-mediated silencing of euchromatic genes by KRAB zinc-finger proteins.

Schultz DC, Ayyanathan K, Negorev D, Maul GG, Rauscher FJ

Posttranslational modification of histones has emerged as a key regulatory signal in eukaryotic gene expression. Recent genetic and biochemical studies link H3-lysine 9 (H3-K9) methylation to HP1-mediated heterochromatin formation and gene silencing. However, the mechanisms that target and coordinate these activities to specific genes is poorly understood. Here we report that the KAP-1 corepressor for the KRAB-ZFP superfamily of transcriptional ... [more]

Genes Dev. Apr. 15, 2002; 16(8);919-32 [Pubmed: 11959841]

Throughput

  • Low Throughput

Curated By

  • BioGRID