RAD5
Gene Ontology Biological Process
Gene Ontology Molecular Function
Gene Ontology Cellular Component
RAD50
Gene Ontology Biological Process
- base-excision repair [IMP]
- double-strand break repair via break-induced replication [TAS]
- double-strand break repair via nonhomologous end joining [IMP]
- meiotic DNA double-strand break formation [TAS]
- meiotic DNA double-strand break processing [TAS]
- meiotic nuclear division [IMP]
- mitochondrial double-strand break repair via homologous recombination [IMP]
- negative regulation of endodeoxyribonuclease activity [IDA]
- telomere maintenance [IMP]
- telomere maintenance via recombination [IMP]
Gene Ontology Molecular Function
Gene Ontology Cellular Component
Dosage Lethality
A genetic interaction is inferred when over expression or increased dosage of one gene causes lethality in a strain that is mutated or deleted for another gene.
Publication
Rad5 dysregulation drives hyperactive recombination at replication forks resulting in cisplatin sensitivity and genome instability.
The postreplication repair gene, HLTF, is often amplified and overexpressed in cancer. Here we model HLTF dysregulation through the functionally conserved Saccharomyces cerevisiae ortholog, RAD5. Genetic interaction profiling and landscape enrichment analysis of RAD5 overexpression (RAD5OE) reveals requirements for genes involved in recombination, crossover resolution, and DNA replication. While RAD5OE and rad5Δ both cause cisplatin sensitivity and share many genetic ... [more]
Throughput
- High Throughput
Ontology Terms
- phenotype: inviable (APO:0000112)
Additional Notes
- over-expression of Rad5 is lethal in combination with the hit gene
Related interactions
Interaction | Experimental Evidence Code | Dataset | Throughput | Score | Curated By | Notes |
---|---|---|---|---|---|---|
RAD5 RAD50 | Synthetic Growth Defect Synthetic Growth Defect A genetic interaction is inferred when mutations in separate genes, each of which alone causes a minimal phenotype, result in a significant growth defect under a given condition when combined in the same cell. | High | - | BioGRID | 457074 | |
RAD50 RAD5 | Synthetic Growth Defect Synthetic Growth Defect A genetic interaction is inferred when mutations in separate genes, each of which alone causes a minimal phenotype, result in a significant growth defect under a given condition when combined in the same cell. | High | - | BioGRID | 457124 | |
RAD50 RAD5 | Synthetic Growth Defect Synthetic Growth Defect A genetic interaction is inferred when mutations in separate genes, each of which alone causes a minimal phenotype, result in a significant growth defect under a given condition when combined in the same cell. | High | - | BioGRID | 451616 |
Curated By
- BioGRID