An interaction is inferred when a bait protein is affinity captured from cell extracts by either polyclonal antibody or epitope tag and the associated interaction partner is identified by mass spectrometric methods.

Publication

BAP1 regulation of the key adaptor protein NCoR1 is critical for Î³-globin gene repression.

Yu L, Jearawiriyapaisarn N, Lee MP, Hosoya T, Wu Q, Myers G, Lim KC, Kurita R, Nakamura Y, Vojtek AB, Rual JF, Engel JD

Human globin gene production transcriptionally "switches" from fetal to adult synthesis shortly after birth and is controlled by macromolecular complexes that enhance or suppress transcription by cis elements scattered throughout the locus. The DRED (direct repeat erythroid-definitive) repressor is recruited to the Îµ-globin and Î³-globin promoters by the orphan nuclear receptors TR2 (NR2C1) and TR4 (NR2C2) to engender their silencing ... [more]

Genes Dev. Dec. 01, 2017; 32(23-24);1537-1549 [Pubmed: 30463901]

Throughput

High Throughput

Additional Notes

assayed using BioID (proximity-dependent biotin identification)

Related interactions

Interaction	Experimental Evidence Code	Dataset	Throughput	Score	Curated By	Notes
DNAJB1 NR2C2	Proximity Label-MS Proximity Label-MS An interaction is inferred when a bait-enzyme fusion protein selectively modifies a vicinal protein with a diffusible reactive product, followed by affinity capture of the modified protein and identification by mass spectrometric methods.	Piette BL (2021)	High	55	BioGRID	2938362

Curated By

BioGRID

Interaction Summary

NR2C2

Gene Ontology Biological Process

Gene Ontology Molecular Function

protein binding [IPI]protein heterodimerization activity [IDA]receptor activity [TAS]sequence-specific DNA binding [IDA]sequence-specific DNA binding transcription factor activity [IDA]transcription coactivator activity [TAS]

Gene Ontology Cellular Component

DNAJB1

Gene Ontology Biological Process

Gene Ontology Molecular Function

ATPase activator activity [IDA]ATPase binding [IPI]Hsp70 protein binding [IPI]chaperone binding [IPI]protein binding [IPI]unfolded protein binding [IDA]