BAIT

CEP97

2810403B08Rik, LRRIQ2
centrosomal protein 97kDa
GO Process (0)
GO Function (1)
GO Component (4)

Gene Ontology Molecular Function

Gene Ontology Cellular Component

Homo sapiens
PREY

KCTD12

C13orf2, PFET1, PFETIN
potassium channel tetramerization domain containing 12
UPS Project
GO Process (0)
GO Function (1)
GO Component (1)

Gene Ontology Molecular Function

Gene Ontology Cellular Component

Homo sapiens

Affinity Capture-Western

An interaction is inferred when a bait protein is affinity captured from cell extracts by either polyclonal antibody or epitope tag and the associated interaction partner identified by Western blot with a specific polyclonal antibody or second epitope tag. This category is also used if an interacting protein is visualized directly by dye stain or radioactivity. Note that this differs from any co-purification experiment involving affinity capture in that the co-purification experiment involves at least one extra purification step to get rid of potential contaminating proteins.

Publication

Cullin-3-KCTD10-mediated CEP97 degradation promotes primary cilium formation.

Nagai T, Mukoyama S, Kagiwada H, Goshima N, Mizuno K

Primary cilia are antenna-like sensory organelles that transmit various extracellular signals. Ciliogenesis requires the removal of CP110 and its interactor CEP97 from the mother centriole for initiating ciliary axoneme extension, but the underlying mechanism remains unknown. Here we show that, upon serum starvation, CEP97 is partially degraded by the ubiquitin-proteasome system. CEP97 was polyubiquitylated in serum-starved cells, and overexpression of ... [more]

J. Cell. Sci. Dec. 12, 2017; 131(24); [Pubmed: 30404837]

Throughput

  • Low Throughput

Additional Notes

  • assayed using VIP (visible immunoprecipitation)

Curated By

  • BioGRID