BAIT
TNF
DIF, TNF-a, TNF-alpha, TNFSF2, TNFalpha, Tnfa, Tnfsf1a
tumor necrosis factor
GO Process (119)
GO Function (6)
GO Component (11)
Gene Ontology Biological Process
- JNK cascade [IGI]
- MAPK cascade [ISO]
- activation of MAPK activity [ISO]
- activation of MAPKKK activity [ISO]
- activation of cysteine-type endopeptidase activity involved in apoptotic process [IDA, ISO]
- apoptotic process [ISO]
- apoptotic signaling pathway [IDA]
- calcium-mediated signaling [ISO]
- cell activation [ISO]
- cell proliferation [TAS]
- cellular extravasation [IDA]
- cellular response to amino acid stimulus [IDA]
- cellular response to lipopolysaccharide [IDA]
- cellular response to nicotine [ISO]
- cellular response to organic cyclic compound [ISO]
- chronic inflammatory response to antigenic stimulus [ISO]
- defense response [IMP]
- defense response to Gram-positive bacterium [IGI]
- defense response to bacterium [IDA]
- detection of mechanical stimulus involved in sensory perception of pain [ISO]
- epithelial cell proliferation involved in salivary gland morphogenesis [IDA]
- extracellular matrix organization [IDA]
- extrinsic apoptotic signaling pathway [IGI, ISO]
- extrinsic apoptotic signaling pathway via death domain receptors [IGI, ISO]
- glucose metabolic process [IDA]
- humoral immune response [IMP]
- inflammatory response [IGI, ISO, TAS]
- intrinsic apoptotic signaling pathway in response to DNA damage [IDA]
- leukocyte migration [IDA]
- leukocyte tethering or rolling [ISO]
- lipopolysaccharide-mediated signaling pathway [ISO]
- multicellular organismal development [IMP]
- necroptotic signaling pathway [IGI, ISO]
- negative regulation of L-glutamate transport [ISO]
- negative regulation of alkaline phosphatase activity [IDA]
- negative regulation of branching involved in lung morphogenesis [ISO]
- negative regulation of cell proliferation [ISO]
- negative regulation of cytokine secretion involved in immune response [ISO]
- negative regulation of extrinsic apoptotic signaling pathway in absence of ligand [ISO]
- negative regulation of gene expression [ISO]
- negative regulation of glucose import [IDA, ISO]
- negative regulation of growth of symbiont in host [IGI]
- negative regulation of interleukin-6 production [ISO]
- negative regulation of lipid catabolic process [ISO]
- negative regulation of myoblast differentiation [IMP]
- negative regulation of osteoblast differentiation [IDA]
- negative regulation of protein complex disassembly [ISO]
- negative regulation of transcription from RNA polymerase II promoter [IDA, ISO]
- negative regulation of transcription, DNA-templated [ISO]
- negative regulation of viral genome replication [ISO]
- organ morphogenesis [IMP]
- osteoclast differentiation [IGI]
- positive regulation of I-kappaB kinase/NF-kappaB signaling [IDA, IGI, ISO]
- positive regulation of JNK cascade [IDA, IGI]
- positive regulation of JUN kinase activity [ISO]
- positive regulation of MAP kinase activity [ISO]
- positive regulation of NF-kappaB import into nucleus [IMP, ISO]
- positive regulation of NF-kappaB transcription factor activity [ISO]
- positive regulation of NFAT protein import into nucleus [ISO]
- positive regulation of action potential [ISO]
- positive regulation of apoptotic process [ISO]
- positive regulation of calcidiol 1-monooxygenase activity [ISO]
- positive regulation of cell proliferation [TAS]
- positive regulation of chemokine (C-X-C motif) ligand 2 production [ISO]
- positive regulation of chemokine biosynthetic process [ISO]
- positive regulation of chemokine production [ISO]
- positive regulation of chronic inflammatory response to antigenic stimulus [IGI]
- positive regulation of cysteine-type endopeptidase activity involved in apoptotic process [ISO]
- positive regulation of cytokine production [ISO]
- positive regulation of cytokine secretion [ISO]
- positive regulation of fever generation [IDA]
- positive regulation of gene expression [ISO]
- positive regulation of hair follicle development [IMP]
- positive regulation of heterotypic cell-cell adhesion [ISO]
- positive regulation of humoral immune response mediated by circulating immunoglobulin [IGI]
- positive regulation of inflammatory response [ISO]
- positive regulation of interferon-gamma production [IGI]
- positive regulation of interleukin-18 production [ISO]
- positive regulation of interleukin-6 production [IDA]
- positive regulation of interleukin-8 biosynthetic process [ISO]
- positive regulation of membrane protein ectodomain proteolysis [ISO]
- positive regulation of mitosis [ISO]
- positive regulation of neuron apoptotic process [ISO]
- positive regulation of nitric oxide biosynthetic process [ISO]
- positive regulation of osteoclast differentiation [ISO]
- positive regulation of peptidyl-serine phosphorylation [ISO]
- positive regulation of podosome assembly [ISO]
- positive regulation of programmed cell death [ISO]
- positive regulation of protein complex assembly [ISO]
- positive regulation of protein complex disassembly [ISO]
- positive regulation of protein kinase B signaling [IDA]
- positive regulation of protein localization to cell surface [ISO]
- positive regulation of protein phosphorylation [ISO]
- positive regulation of protein transport [ISO]
- positive regulation of sequence-specific DNA binding transcription factor activity [ISO]
- positive regulation of smooth muscle cell proliferation [ISO]
- positive regulation of synaptic transmission [ISO]
- positive regulation of transcription from RNA polymerase II promoter [IDA, IGI, ISO]
- positive regulation of transcription, DNA-templated [IDA, ISO]
- positive regulation of translational initiation by iron [IDA]
- positive regulation of vitamin D biosynthetic process [ISO]
- protein import into nucleus, translocation [ISO]
- protein kinase B signaling [ISO]
- receptor biosynthetic process [ISO]
- regulation of I-kappaB kinase/NF-kappaB signaling [ISO]
- regulation of branching involved in salivary gland morphogenesis [IDA, IMP]
- regulation of cell proliferation [IDA]
- regulation of immunoglobulin secretion [IDA]
- regulation of insulin secretion [ISO]
- regulation of osteoclast differentiation [IDA]
- regulation of protein phosphorylation [IDA]
- regulation of protein secretion [IDA]
- regulation of reactive oxygen species metabolic process [IDA]
- response to glucocorticoid [ISO]
- response to virus [ISO]
- sequestering of triglyceride [ISO]
- signal transduction [ISO]
- transformed cell apoptotic process [ISO, TAS]
- tumor necrosis factor-mediated signaling pathway [IDA, ISO]
Gene Ontology Molecular Function
Gene Ontology Cellular Component
- cell surface [ISO]
- external side of plasma membrane [IDA]
- extracellular space [IDA, ISO]
- integral component of membrane [ISO]
- integral component of plasma membrane [ISO, TAS]
- intracellular [IDA, IGI]
- membrane raft [IDA, ISO]
- phagocytic cup [IDA]
- plasma membrane [IDA]
- recycling endosome [IDA]
- secretory granule [TAS]
Mus musculus
PREY
TBK1
1200008B05Rik, AI462036, AW048562
TANK-binding kinase 1
GO Process (8)
GO Function (3)
GO Component (1)
Gene Ontology Biological Process
- activation of innate immune response [IMP]
- defense response to Gram-positive bacterium [IMP]
- dendritic cell proliferation [IGI]
- negative regulation of gene expression [IMP]
- positive regulation of interferon-alpha production [ISO]
- positive regulation of interferon-beta biosynthetic process [IMP]
- positive regulation of interferon-beta production [ISO]
- positive regulation of transcription from RNA polymerase II promoter [ISO]
Gene Ontology Molecular Function
Mus musculus
Affinity Capture-Western
An interaction is inferred when a bait protein is affinity captured from cell extracts by either polyclonal antibody or epitope tag and the associated interaction partner identified by Western blot with a specific polyclonal antibody or second epitope tag. This category is also used if an interacting protein is visualized directly by dye stain or radioactivity. Note that this differs from any co-purification experiment involving affinity capture in that the co-purification experiment involves at least one extra purification step to get rid of potential contaminating proteins.
Publication
TBK1 and IKKε prevent TNF-induced cell death by RIPK1 phosphorylation.
The linear-ubiquitin chain assembly complex (LUBAC) modulates signalling via various immune receptors. In tumour necrosis factor (TNF) signalling, linear (also known as M1) ubiquitin enables full gene activation and prevents cell death. However, the mechanisms underlying cell death prevention remain ill-defined. Here, we show that LUBAC activity enables TBK1 and IKKε recruitment to and activation at the TNF receptor 1 ... [more]
Nat. Cell Biol. Dec. 01, 2017; 20(12);1389-1399 [Pubmed: 30420664]
Throughput
- Low Throughput
Curated By
- BioGRID