Affinity Capture-MS

An interaction is inferred when a bait protein is affinity captured from cell extracts by either polyclonal antibody or epitope tag and the associated interaction partner is identified by mass spectrometric methods.

Publication

Ubiquitin-Independent Disassembly by a p97 AAA-ATPase Complex Drives PP1 Holoenzyme Formation.

Weith M, Seiler J, van den Boom J, Kracht M, Huelsmann J, Primorac I, Del Pino Garcia J, Kaschani F, Kaiser M, Musacchio A, Bollen M, Meyer H

The functional diversity of protein phosphatase-1 (PP1), with its countless substrates, relies on the ordered assembly of alternative PP1 holoenzymes. Here, we show that newly synthesized PP1 is first held by its partners SDS22 and inhibitor-3 (I3) in an inactive complex, which needs to be disassembled by the p97 AAA-ATPase to promote exchange to substrate specifiers. Unlike p97-mediated degradative processes ... [more]

Mol. Cell Dec. 15, 2017; 72(4);766-777.e6 [Pubmed: 30344098]

Throughput

  • High Throughput

Curated By

  • BioGRID