RAD51
Gene Ontology Biological Process
- ATP catabolic process [ISO]
- DNA repair [TAS]
- DNA unwinding involved in DNA replication [ISO]
- cellular response to DNA damage stimulus [ISO, TAS]
- cellular response to camptothecin [ISO]
- cellular response to ionizing radiation [ISO]
- double-strand break repair via homologous recombination [IDA, IMP, ISO]
- meiotic nuclear division [IDA]
- positive regulation of DNA ligation [ISO]
- protein homooligomerization [ISO]
- reciprocal meiotic recombination [TAS]
- regulation of double-strand break repair via homologous recombination [ISO]
Gene Ontology Molecular Function
Gene Ontology Cellular Component
TUBG1
Gene Ontology Biological Process
Gene Ontology Molecular Function
Gene Ontology Cellular Component
- apical part of cell [IDA]
- cell leading edge [IDA]
- centriole [IDA]
- centrosome [IDA, ISO]
- ciliary basal body [IDA]
- cilium [IDA]
- condensed nuclear chromosome [IDA]
- cytoplasm [IDA, ISO]
- cytoplasmic microtubule [IDA]
- gamma-tubulin complex [ISO]
- microtubule organizing center [TAS]
- nonmotile primary cilium [IDA]
- pericentriolar material [IDA]
- polar microtubule [ISO]
- recycling endosome [ISO]
- spindle microtubule [IDA]
- spindle pole [TAS]
Affinity Capture-Western
An interaction is inferred when a bait protein is affinity captured from cell extracts by either polyclonal antibody or epitope tag and the associated interaction partner identified by Western blot with a specific polyclonal antibody or second epitope tag. This category is also used if an interacting protein is visualized directly by dye stain or radioactivity. Note that this differs from any co-purification experiment involving affinity capture in that the co-purification experiment involves at least one extra purification step to get rid of potential contaminating proteins.
Publication
DNA damage induce gamma-tubulin-RAD51 nuclear complexes in mammalian cells.
Rad51 protein plays an essential role in recombination repair of DNA double-strand breaks and DNA crosslinking adducts. It is part of complexes which can vary with the stage of the cell cycle and the nature of the DNA lesions. During a search for Rad51-associated proteins in CHO nuclear extracts of S-phase cells by mass spectrometry of proteins immunoprecipitated with Rad51 ... [more]
Throughput
- Low Throughput
Curated By
- BioGRID