BAIT

LIN-2

CELE_F17E5.1
Protein LIN-2
GO Process (4)
GO Function (2)
GO Component (1)

Gene Ontology Biological Process

Gene Ontology Molecular Function

Gene Ontology Cellular Component

Caenorhabditis elegans
PREY

H05L14.2

CELE_H05L14.2
Protein H05L14.2
GO Process (1)
GO Function (0)
GO Component (0)

Gene Ontology Biological Process

Caenorhabditis elegans

Two-hybrid

Bait protein expressed as a DNA binding domain (DBD) fusion and prey expressed as a transcriptional activation domain (TAD) fusion and interaction measured by reporter gene activation.

Publication

A combined binary interaction and phenotypic map of C. elegans cell polarity proteins.

Koorman T, Klompstra D, van der Voet M, Lemmens I, Ramalho JJ, Nieuwenhuize S, van den Heuvel S, Tavernier J, Nance J, Boxem M

The establishment of cell polarity is an essential process for the development of multicellular organisms and the functioning of cells and tissues. Here, we combine large-scale protein interaction mapping with systematic phenotypic profiling to study the network of physical interactions that underlies polarity establishment and maintenance in the nematode Caenorhabditis elegans. Using a fragment-based yeast two-hybrid strategy, we identified 439 interactions ... [more]

Nat. Cell Biol. Mar. 01, 2016; 18(3);337-46 [Pubmed: 26780296]

Throughput

  • High Throughput

Additional Notes

  • Fragment bait constructs

Curated By

  • BioGRID