BAIT
PUM2
PUMH2, PUML2
pumilio RNA-binding family member 2
GO Process (0)
GO Function (2)
GO Component (4)
Gene Ontology Molecular Function
Gene Ontology Cellular Component
Homo sapiens
PREY
SIAH1
SIAH1A
siah E3 ubiquitin protein ligase 1
GO Process (11)
GO Function (5)
GO Component (4)
Gene Ontology Biological Process
- anatomical structure morphogenesis [TAS]
- apoptotic process [TAS]
- axon guidance [TAS]
- nervous system development [TAS]
- neuron apoptotic process [ISS]
- positive regulation of apoptotic process [IDA]
- positive regulation of intrinsic apoptotic signaling pathway [IMP]
- proteasome-mediated ubiquitin-dependent protein catabolic process [ISS]
- protein catabolic process [IDA]
- protein ubiquitination involved in ubiquitin-dependent protein catabolic process [ISS]
- ubiquitin-dependent protein catabolic process [IDA]
Gene Ontology Molecular Function
Homo sapiens
Affinity Capture-RNA
An interaction is inferred when a bait protein is affinity captured from cell extracts by either polyclonal antibody or epitope tag and associated RNA species identified by Northern blot, RT-PCR, affinity labeling, sequencing, or microarray analysis.
Publication
PUM1 and PUM2 exhibit different modes of regulation for SIAH1 that involve cooperativity with NANOS paralogues.
Pumilio (PUM) proteins are RNA-binding proteins that posttranscriptionally regulate gene expression in many organisms. Their PUF domain recognizes specific PUM-binding elements (PBE) in the 3' untranslated region of target mRNAs while engaging protein cofactors such as NANOS that repress the expression of target mRNAs through the recruitment of effector complexes. Although the general process whereby PUM recognizes individual mRNAs has ... [more]
Cell. Mol. Life Sci. Jan. 01, 2019; 76(1);147-161 [Pubmed: 30269240]
Throughput
- Low Throughput
Additional Notes
- assayed using RIP (RNA immunoprecipitation) in which PUM1 or PUM2 proteins were immunoprecipitated and SIAH1 mRNA was detected by RT-PCR
Curated By
- BioGRID