Protein-RNA

An interaction is detected between and protein and an RNA in vitro.

Publication

PUM1 and PUM2 exhibit different modes of regulation for SIAH1 that involve cooperativity with NANOS paralogues.

Sajek M, Janecki DM, Smialek MJ, Ginter-Matuszewska B, Spik A, Oczkowski S, Ilaslan E, Kusz-Zamelczyk K, Kotecki M, Blazewicz J, Jaruzelska J

Pumilio (PUM) proteins are RNA-binding proteins that posttranscriptionally regulate gene expression in many organisms. Their PUF domain recognizes specific PUM-binding elements (PBE) in the 3' untranslated region of target mRNAs while engaging protein cofactors such as NANOS that repress the expression of target mRNAs through the recruitment of effector complexes. Although the general process whereby PUM recognizes individual mRNAs has ... [more]

Cell. Mol. Life Sci. Jan. 01, 2019; 76(1);147-161 [Pubmed: 30269240]

Throughput

  • Low Throughput

Additional Notes

  • assayed using EMSA (electrophoretic mobility shift assay) in which NANOS2 proteins shifts SIAH1 mRNA

Curated By

  • BioGRID