BAIT

T11B7.1

CELE_T11B7.1
Protein T11B7.1
GO Process (0)
GO Function (1)
GO Component (0)

Gene Ontology Molecular Function

Caenorhabditis elegans
PREY

F55H2.7

CELE_F55H2.7
F55H2.7 encodes an unfamiliar protein that is dispensable for viability and gross morphology in mass RNAi screens; it residues in a common operon with clu-1, and thus might act in concert with it.
GO Process (0)
GO Function (0)
GO Component (0)
Caenorhabditis elegans

Two-hybrid

Bait protein expressed as a DNA binding domain (DBD) fusion and prey expressed as a transcriptional activation domain (TAD) fusion and interaction measured by reporter gene activation.

Publication

Empirically controlled mapping of the Caenorhabditis elegans protein-protein interactome network.

Simonis N, Rual JF, Carvunis AR, Tasan M, Lemmens I, Hirozane-Kishikawa T, Hao T, Sahalie JM, Venkatesan K, Gebreab F, Cevik S, Klitgord N, Fan C, Braun P, Li N, Ayivi-Guedehoussou N, Dann E, Bertin N, Szeto D, Dricot A, Yildirim MA, Lin C, de Smet AS, Kao HL, Simon C, Smolyar A, Ahn JS, Tewari M, Boxem M, Milstein S, Yu H, Dreze M, Vandenhaute J, Gunsalus KC, Cusick ME, Hill DE, Tavernier J, Roth FP, Vidal M

To provide accurate biological hypotheses and elucidate global properties of cellular networks, systematic identification of protein-protein interactions must meet high quality standards.We present an expanded C. elegans protein-protein interaction network, or 'interactome' map, derived from testing a matrix of approximately 10,000 x approximately 10,000 proteins using a highly specific, high-throughput yeast two-hybrid system. Through a new empirical quality control framework, ... [more]

Nat. Methods Jan. 01, 2009; 6(1);47-54 [Pubmed: 19123269]

Throughput

  • High Throughput

Curated By

  • BioGRID