BAIT

RPB5

SPAC23C4.15
DNA-directed RNA polymerase I, II and III subunit Rpb5
Schizosaccharomyces pombe (972h)
PREY

TFA2

SPCC1672.08c
transcription factor TFIIE beta subunit, TFIIEB, Tfa2
GO Process (1)
GO Function (2)
GO Component (3)

Gene Ontology Biological Process

Gene Ontology Molecular Function

Gene Ontology Cellular Component

Schizosaccharomyces pombe (972h)

Affinity Capture-Western

An interaction is inferred when a bait protein is affinity captured from cell extracts by either polyclonal antibody or epitope tag and the associated interaction partner identified by Western blot with a specific polyclonal antibody or second epitope tag. This category is also used if an interacting protein is visualized directly by dye stain or radioactivity. Note that this differs from any co-purification experiment involving affinity capture in that the co-purification experiment involves at least one extra purification step to get rid of potential contaminating proteins.

Publication

Studies of Schizosaccharomyces pombe TFIIE indicate conformational and functional changes in RNA polymerase II at transcription initiation.

Hayashi K, Watanabe T, Tanaka A, Furumoto T, Sato-Tsuchiya C, Kimura M, Yokoi M, Ishihama A, Hanaoka F, Ohkuma Y

The general transcription factor TFIIE plays essential roles in transcription by RNA polymerase II (PolII). Despite recent progress, the elucidation of its precise mechanisms including biological functions awaits further characterization. We report the isolation and characterization of Schizosaccharomyces pombe TFIIE (spTFIIE). Like human and other eukaryotic TFIIE proteins, spTFIIE consists of alpha and beta subunits and the genes encoding both ... [more]

Genes Cells Mar. 01, 2005; 10(3);207-24 [Pubmed: 15743411]

Throughput

  • Low Throughput

Curated By

  • BioGRID