SAE2
Gene Ontology Biological Process
- DNA catabolic process, endonucleolytic [IDA, IMP]
- DNA double-strand break processing [IGI]
- DNA double-strand break processing involved in repair via synthesis-dependent strand annealing [IMP]
- gene conversion at mating-type locus, DNA double-strand break processing [IMP]
- meiotic DNA double-strand break formation [IGI]
- meiotic DNA double-strand break processing [IGI, IMP]
- telomere maintenance [IMP]
- telomeric 3' overhang formation [IMP]
Gene Ontology Molecular Function
RAD50
Gene Ontology Biological Process
- base-excision repair [IMP]
- double-strand break repair via break-induced replication [TAS]
- double-strand break repair via nonhomologous end joining [IMP]
- meiotic DNA double-strand break formation [TAS]
- meiotic DNA double-strand break processing [TAS]
- meiotic nuclear division [IMP]
- mitochondrial double-strand break repair via homologous recombination [IMP]
- negative regulation of endodeoxyribonuclease activity [IDA]
- telomere maintenance [IMP]
- telomere maintenance via recombination [IMP]
Gene Ontology Molecular Function
Gene Ontology Cellular Component
Phenotypic Suppression
A genetic interaction is inferred when mutation or over expression of one gene results in suppression of any phenotype (other than lethality/growth defect) associated with mutation or over expression of another gene.
Publication
The ATP-bound conformation of the Mre11-Rad50 complex is essential for Tel1/ATM activation.
Activation of the checkpoint protein Tel1 requires the Mre11-Rad50-Xrs2 (MRX) complex, which recruits Tel1 at DNA double-strand breaks (DSBs) through direct interaction between Tel1 and Xrs2. However, in vitro Tel1 activation by MRX requires ATP binding to Rad50, suggesting a role also for the MR subcomplex in Tel1 activation. Here we describe two separation-of-functions alleles, mre11-S499P and rad50-A78T, which we ... [more]
Throughput
- Low Throughput
Ontology Terms
- phenotype: resistance to chemicals (APO:0000087)
- phenotype: protein/peptide modification (APO:0000131)
- phenotype: cell size (APO:0000052)
Additional Notes
- deletion of tel1 or mutation of mre11 or rad50 inhibits the rescue of sae2 sensitivity to HU (CHEBI:44423) and MMS (CHEBI:25255)
- deletion rescues the sae2 adaptation defect
Related interactions
| Interaction | Experimental Evidence Code | Dataset | Throughput | Score | Curated By | Notes |
|---|---|---|---|---|---|---|
| RAD50 SAE2 | Dosage Growth Defect Dosage Growth Defect A genetic interaction is inferred when over expression or increased dosage of one gene causes a growth defect in a strain that is mutated or deleted for another gene. | Low | - | BioGRID | 519354 | |
| RAD50 SAE2 | Dosage Lethality Dosage Lethality A genetic interaction is inferred when over expression or increased dosage of one gene causes lethality in a strain that is mutated or deleted for another gene. | Low | - | BioGRID | 519348 | |
| SAE2 RAD50 | Phenotypic Suppression Phenotypic Suppression A genetic interaction is inferred when mutation or over expression of one gene results in suppression of any phenotype (other than lethality/growth defect) associated with mutation or over expression of another gene. | Low | - | BioGRID | 3199780 | |
| SAE2 RAD50 | Reconstituted Complex Reconstituted Complex An interaction is detected between purified proteins in vitro. | Low | - | BioGRID | - | |
| SAE2 RAD50 | Reconstituted Complex Reconstituted Complex An interaction is detected between purified proteins in vitro. | Low | - | BioGRID | - | |
| RAD50 SAE2 | Reconstituted Complex Reconstituted Complex An interaction is detected between purified proteins in vitro. | Low | - | BioGRID | - | |
| RAD50 SAE2 | Reconstituted Complex Reconstituted Complex An interaction is detected between purified proteins in vitro. | Low | - | BioGRID | - | |
| RAD50 SAE2 | Synthetic Growth Defect Synthetic Growth Defect A genetic interaction is inferred when mutations in separate genes, each of which alone causes a minimal phenotype, result in a significant growth defect under a given condition when combined in the same cell. | Low | - | BioGRID | 2344236 | |
| SAE2 RAD50 | Synthetic Rescue Synthetic Rescue A genetic interaction is inferred when mutations or deletions of one gene rescues the lethality or growth defect of a strain mutated or deleted for another gene. | Low | - | BioGRID | 3403605 | |
| RAD50 SAE2 | Two-hybrid Two-hybrid Bait protein expressed as a DNA binding domain (DBD) fusion and prey expressed as a transcriptional activation domain (TAD) fusion and interaction measured by reporter gene activation. | Low | - | BioGRID | - |
Curated By
- BioGRID