BMP7
Gene Ontology Biological Process
- BMP signaling pathway [IDA]
- SMAD protein signal transduction [IDA]
- cellular response to hypoxia [ISS]
- dendrite development [TAS]
- epithelial to mesenchymal transition [TAS]
- extracellular matrix organization [TAS]
- mesenchymal cell differentiation [IDA]
- mesenchyme development [ISS]
- mesonephros development [IEP]
- metanephros development [IEP]
- monocyte aggregation [IDA]
- negative regulation of MAP kinase activity [IDA]
- negative regulation of NF-kappaB import into nucleus [ISS]
- negative regulation of NF-kappaB transcription factor activity [ISS]
- negative regulation of cell cycle [IDA]
- negative regulation of cell death [IDA]
- negative regulation of glomerular mesangial cell proliferation [IDA]
- negative regulation of mitosis [IDA]
- negative regulation of neuron differentiation [IDA]
- negative regulation of phosphorylation [IDA]
- negative regulation of striated muscle cell apoptotic process [ISS]
- negative regulation of transcription, DNA-templated [IDA]
- neuron projection morphogenesis [IDA]
- positive regulation of bone mineralization [IDA]
- positive regulation of dendrite development [IDA]
- positive regulation of heterotypic cell-cell adhesion [IDA]
- positive regulation of hyaluranon cable assembly [IDA]
- positive regulation of osteoblast differentiation [IDA]
- positive regulation of pathway-restricted SMAD protein phosphorylation [IDA, TAS]
- positive regulation of peptidyl-threonine phosphorylation [IDA]
- positive regulation of transcription, DNA-templated [IDA]
- protein localization to nucleus [IDA]
- regulation of apoptotic process [IBA]
- regulation of pathway-restricted SMAD protein phosphorylation [IDA]
- regulation of removal of superoxide radicals [ISS]
- skeletal system development [TAS]
- steroid hormone mediated signaling pathway [IMP]
- ureteric bud development [ISS]
Gene Ontology Molecular Function
Gene Ontology Cellular Component
UBE4A
Gene Ontology Molecular Function
Affinity Capture-Western
An interaction is inferred when a bait protein is affinity captured from cell extracts by either polyclonal antibody or epitope tag and the associated interaction partner identified by Western blot with a specific polyclonal antibody or second epitope tag. This category is also used if an interacting protein is visualized directly by dye stain or radioactivity. Note that this differs from any co-purification experiment involving affinity capture in that the co-purification experiment involves at least one extra purification step to get rid of potential contaminating proteins.
Publication
Bone morphogenetic protein (BMP) 7 expression is regulated by the E3 ligase UBE4A in diabetic nephropathy.
Mesangial cells played a central role in the pathophysiology of diabetic nephropathy (DN). Our goal was to evaluate the molecular mechanism that regulates loss of BMP7 protein expression in DN. The mRNA and protein levels of BMP7 or UBE4A were detected using qRT-PCR and Western blot respectively. Mass spectrometry and co-immunoprecipitation were used to explore the E3 ligase which regulated ... [more]
Throughput
- Low Throughput
Related interactions
| Interaction | Experimental Evidence Code | Dataset | Throughput | Score | Curated By | Notes |
|---|---|---|---|---|---|---|
| BMP7 UBE4A | Affinity Capture-MS Affinity Capture-MS An interaction is inferred when a bait protein is affinity captured from cell extracts by either polyclonal antibody or epitope tag and the associated interaction partner is identified by mass spectrometric methods. | High | - | BioGRID | - |
Curated By
- BioGRID