BAIT
CSK
c-src tyrosine kinase
GO Process (18)
GO Function (6)
GO Component (5)
Gene Ontology Biological Process
- T cell costimulation [TAS]
- T cell receptor signaling pathway [TAS]
- adherens junction organization [IBA]
- blood coagulation [TAS]
- cell differentiation [IBA]
- cell migration [IBA]
- central nervous system development [IBA]
- epidermal growth factor receptor signaling pathway [TAS]
- innate immune response [IBA]
- morphogenesis of an epithelium [IBA]
- negative regulation of Golgi to plasma membrane protein transport [IDA]
- peptidyl-tyrosine autophosphorylation [IBA]
- platelet activation [TAS]
- protein phosphorylation [TAS]
- regulation of Fc receptor mediated stimulatory signaling pathway [IBA]
- regulation of cell proliferation [IBA]
- regulation of cytokine production [IBA]
- transmembrane receptor protein tyrosine kinase signaling pathway [IBA]
Gene Ontology Molecular Function
Gene Ontology Cellular Component
Homo sapiens
PREY
JAK2
JTK10, THCYT3
Janus kinase 2
GO Process (49)
GO Function (12)
GO Component (7)
Gene Ontology Biological Process
- JAK-STAT cascade [TAS]
- JAK-STAT cascade involved in growth hormone signaling pathway [ISS, TAS]
- STAT protein import into nucleus [ISS]
- actin filament polymerization [NAS]
- activation of JAK2 kinase activity [ISS]
- activation of cysteine-type endopeptidase activity involved in apoptotic process [ISS]
- activation of cysteine-type endopeptidase activity involved in apoptotic signaling pathway [ISS]
- apoptotic process [ISS]
- blood coagulation [TAS]
- cell differentiation [ISS]
- cell migration [IBA]
- cellular component movement [TAS]
- cytokine-mediated signaling pathway [IDA, ISS, TAS]
- enzyme linked receptor protein signaling pathway [ISS]
- erythrocyte differentiation [IBA, ISS]
- extrinsic apoptotic signaling pathway [ISS]
- growth hormone receptor signaling pathway [IDA]
- histone H3-Y41 phosphorylation [IDA]
- innate immune response [IBA]
- interferon-gamma-mediated signaling pathway [TAS]
- interleukin-12-mediated signaling pathway [IDA]
- intracellular signal transduction [ISS]
- mammary gland epithelium development [ISS]
- mesoderm development [TAS]
- negative regulation of DNA binding [ISS]
- negative regulation of cell proliferation [ISS]
- peptidyl-tyrosine autophosphorylation [IBA]
- peptidyl-tyrosine phosphorylation [ISS]
- positive regulation of cell-substrate adhesion [IDA]
- positive regulation of growth hormone receptor signaling pathway [ISS]
- positive regulation of nitric-oxide synthase biosynthetic process [ISS]
- positive regulation of peptidyl-tyrosine phosphorylation [ISS]
- positive regulation of phosphatidylinositol 3-kinase signaling [ISS]
- positive regulation of tumor necrosis factor production [ISS]
- positive regulation of tyrosine phosphorylation of Stat3 protein [ISS]
- positive regulation of tyrosine phosphorylation of Stat5 protein [ISS]
- protein autophosphorylation [ISS]
- protein phosphorylation [TAS]
- regulation of apoptotic process [IBA]
- regulation of cell proliferation [IBA]
- regulation of inflammatory response [IDA]
- regulation of interferon-gamma-mediated signaling pathway [TAS]
- response to antibiotic [IDA]
- response to interleukin-12 [IDA]
- response to lipopolysaccharide [ISS]
- response to tumor necrosis factor [IDA]
- signal transduction [ISS]
- tumor necrosis factor-mediated signaling pathway [IDA]
- tyrosine phosphorylation of STAT protein [IBA, ISS]
Gene Ontology Molecular Function- SH2 domain binding [IPI]
- growth hormone receptor binding [IBA, ISS]
- heme binding [IDA]
- histone binding [IDA]
- histone kinase activity (H3-Y41 specific) [IDA]
- interleukin-12 receptor binding [ISS]
- non-membrane spanning protein tyrosine kinase activity [IBA]
- protein binding [IPI]
- protein kinase activity [NAS]
- protein kinase binding [IDA]
- protein tyrosine kinase activity [EXP, ISS, TAS]
- receptor binding [IPI]
- SH2 domain binding [IPI]
- growth hormone receptor binding [IBA, ISS]
- heme binding [IDA]
- histone binding [IDA]
- histone kinase activity (H3-Y41 specific) [IDA]
- interleukin-12 receptor binding [ISS]
- non-membrane spanning protein tyrosine kinase activity [IBA]
- protein binding [IPI]
- protein kinase activity [NAS]
- protein kinase binding [IDA]
- protein tyrosine kinase activity [EXP, ISS, TAS]
- receptor binding [IPI]
Gene Ontology Cellular Component
Homo sapiens
Synthetic Lethality
A genetic interaction is inferred when mutations or deletions in separate genes, each of which alone causes a minimal phenotype, result in lethality when combined in the same cell under a given condition.
Publication
Estrogen-regulated feedback loop limits the efficacy of estrogen receptor-targeted breast cancer therapy.
Endocrine therapy resistance invariably develops in advanced estrogen receptor-positive (ER+) breast cancer, but the underlying mechanisms are largely unknown. We have identified C-terminal SRC kinase (CSK) as a critical node in a previously unappreciated negative feedback loop that limits the efficacy of current ER-targeted therapies. Estrogen directly drives CSK expression in ER+ breast cancer. At low CSK levels, as is ... [more]
Proc. Natl. Acad. Sci. U.S.A. Dec. 31, 2017; 115(31);7869-7878 [Pubmed: 29987050]
Throughput
- High Throughput
Ontology Terms
- phenotype: growth abnormality (HP:0001507)
- phenotype: viability (PATO:0000169)
Additional Notes
- CRISPR GI screen
- Cell Line:T47D
- Experimental Setup: Timecourse, negative selection, viability
- GIST: A-phenotypic negative genetic interaction
- Library: Geckov2
- Significance Threshold: FDR<0.05
Curated By
- BioGRID