BAIT
GBF1
ARF1GEF
golgi brefeldin A resistant guanine nucleotide exchange factor 1
GO Process (6)
GO Function (2)
GO Component (4)
Gene Ontology Biological Process
Gene Ontology Molecular Function
Gene Ontology Cellular Component
Homo sapiens
PREY
CTTN
EMS1
cortactin
GO Process (9)
GO Function (1)
GO Component (12)
Gene Ontology Biological Process
- actin cytoskeleton reorganization [ISS]
- cell motility [ISS]
- focal adhesion assembly [ISS]
- intracellular protein transport [IMP]
- lamellipodium organization [ISS]
- neuron projection morphogenesis [ISS]
- positive regulation of actin filament polymerization [ISS]
- receptor-mediated endocytosis [ISS]
- regulation of axon extension [ISS]
Gene Ontology Molecular Function
Gene Ontology Cellular Component
Homo sapiens
Proximity Label-MS
An interaction is inferred when a bait-enzyme fusion protein selectively modifies a vicinal protein with a diffusible reactive product, followed by affinity capture of the modified protein and identification by mass spectrometric methods.
Publication
BioID Performed on Golgi Enriched Fractions Identify C10orf76 as a GBF1 Binding Protein Essential for Golgi Maintenance and Secretion.
The Golgi-specific Brefeldin-A resistance factor 1 (GBF1) is the only large GEF that regulates Arf activation at the cis-Golgi and is actively recruited to membranes on an increase in Arf-GDP. Recent studies have revealed that GBF1 recruitment requires one or more heat-labile and protease-sensitive protein factor(s) (Quilty et al., 2018, J. Cell Science, 132). Proximity-dependent biotinylation (BioID) and mass spectrometry ... [more]
Mol. Cell Proteomics Nov. 01, 2019; 18(11);2285-2297 [Pubmed: 31519766]
Throughput
- High Throughput
Additional Notes
- BioID system
- Proteins with two or more unique peptides and having a Bayesian False Discovery Rate of 1% (BFDR less than or equal to 0.01) are high-confidence proximity interactors.
Curated By
- BioGRID