GBF1
Gene Ontology Biological Process
Gene Ontology Molecular Function
Gene Ontology Cellular Component
VCL
Gene Ontology Biological Process
- adherens junction assembly [IMP]
- apical junction assembly [IMP]
- blood coagulation [TAS]
- cell adhesion [TAS]
- cell-matrix adhesion [TAS]
- cellular component movement [TAS]
- epithelial cell-cell adhesion [IMP]
- lamellipodium assembly [ISS]
- morphogenesis of an epithelium [IMP]
- muscle contraction [TAS]
- negative regulation of cell migration [TAS]
- platelet activation [TAS]
- platelet aggregation [IMP]
- platelet degranulation [TAS]
- protein localization to cell surface [IMP]
Gene Ontology Molecular Function
Gene Ontology Cellular Component
Proximity Label-MS
An interaction is inferred when a bait-enzyme fusion protein selectively modifies a vicinal protein with a diffusible reactive product, followed by affinity capture of the modified protein and identification by mass spectrometric methods.
Publication
BioID Performed on Golgi Enriched Fractions Identify C10orf76 as a GBF1 Binding Protein Essential for Golgi Maintenance and Secretion.
The Golgi-specific Brefeldin-A resistance factor 1 (GBF1) is the only large GEF that regulates Arf activation at the cis-Golgi and is actively recruited to membranes on an increase in Arf-GDP. Recent studies have revealed that GBF1 recruitment requires one or more heat-labile and protease-sensitive protein factor(s) (Quilty et al., 2018, J. Cell Science, 132). Proximity-dependent biotinylation (BioID) and mass spectrometry ... [more]
Throughput
- High Throughput
Additional Notes
- BioID system
- Proteins with two or more unique peptides and having a Bayesian False Discovery Rate of 1% (BFDR less than or equal to 0.01) are high-confidence proximity interactors.
Related interactions
| Interaction | Experimental Evidence Code | Dataset | Throughput | Score | Curated By | Notes |
|---|---|---|---|---|---|---|
| VCL GBF1 | Proximity Label-MS Proximity Label-MS An interaction is inferred when a bait-enzyme fusion protein selectively modifies a vicinal protein with a diffusible reactive product, followed by affinity capture of the modified protein and identification by mass spectrometric methods. | High | 45.33 | BioGRID | 3010486 |
Curated By
- BioGRID