Publication

BioID Performed on Golgi Enriched Fractions Identify C10orf76 as a GBF1 Binding Protein Essential for Golgi Maintenance and Secretion.

Chan CJ, Le R, Burns K, Ahmed K, Coyaud E, Laurent EMN, Raught B, Melancon P

The Golgi-specific Brefeldin-A resistance factor 1 (GBF1) is the only large GEF that regulates Arf activation at the cis-Golgi and is actively recruited to membranes on an increase in Arf-GDP. Recent studies have revealed that GBF1 recruitment requires one or more heat-labile and protease-sensitive protein factor(s) (Quilty et al., 2018, J. Cell Science, 132). Proximity-dependent biotinylation (BioID) and mass spectrometry ... [more]

Throughput

• High Throughput

Additional Notes

• BioID system
• Proteins with two or more unique peptides and having a Bayesian False Discovery Rate of 1% (BFDR less than or equal to 0.01) are high-confidence proximity interactors.

Curated By

• BioGRID