BAIT
SMAD3
AU022421, Madh3, RP23-63E8.1
SMAD family member 3
GO Process (73)
GO Function (31)
GO Component (11)
Gene Ontology Biological Process
- SMAD protein complex assembly [ISO]
- SMAD protein signal transduction [IGI]
- T cell activation [IMP]
- activation of cysteine-type endopeptidase activity involved in apoptotic process [ISO]
- activation of cysteine-type endopeptidase activity involved in apoptotic signaling pathway [ISO]
- activin receptor signaling pathway [ISO]
- cell cycle arrest [ISO]
- cell-cell junction organization [ISO]
- developmental growth [IGI]
- embryonic cranial skeleton morphogenesis [IGI]
- embryonic foregut morphogenesis [IGI]
- embryonic pattern specification [IGI]
- endoderm development [IGI]
- evasion or tolerance of host defenses by virus [ISO]
- extrinsic apoptotic signaling pathway [ISO]
- gastrulation [IGI]
- heart looping [IGI]
- immune response [ISO]
- immune system development [IGI]
- in utero embryonic development [IGI]
- intracellular signal transduction [ISO]
- lens fiber cell differentiation [IMP]
- liver development [IGI]
- mesoderm formation [IMP]
- negative regulation of apoptotic process [ISO]
- negative regulation of cell growth [ISO]
- negative regulation of cell proliferation [ISO]
- negative regulation of inflammatory response [IMP]
- negative regulation of mitotic cell cycle [ISO]
- negative regulation of osteoblast differentiation [IGI]
- negative regulation of osteoblast proliferation [IMP]
- negative regulation of protein catabolic process [ISO]
- negative regulation of protein phosphorylation [ISO]
- negative regulation of transcription from RNA polymerase II promoter [IDA, ISO]
- negative regulation of wound healing [IMP]
- nodal signaling pathway [ISO]
- osteoblast development [IGI]
- osteoblast differentiation [IMP]
- paraxial mesoderm morphogenesis [IMP]
- pericardium development [IGI]
- positive regulation of alkaline phosphatase activity [ISO]
- positive regulation of bone mineralization [ISO]
- positive regulation of canonical Wnt signaling pathway [ISO]
- positive regulation of catenin import into nucleus [ISO]
- positive regulation of cell migration [ISO]
- positive regulation of chondrocyte differentiation [IMP]
- positive regulation of epithelial to mesenchymal transition [ISO]
- positive regulation of focal adhesion assembly [ISO]
- positive regulation of gene expression [ISO]
- positive regulation of interleukin-1 beta production [ISO]
- positive regulation of positive chemotaxis [ISO]
- positive regulation of stress fiber assembly [ISO]
- positive regulation of transcription factor import into nucleus [ISO]
- positive regulation of transcription from RNA polymerase II promoter [IDA, IGI, ISO]
- positive regulation of transcription, DNA-templated [IDA, ISO]
- positive regulation of transforming growth factor beta3 production [ISO]
- protein stabilization [ISO]
- regulation of binding [IDA]
- regulation of epithelial cell proliferation [IMP]
- regulation of immune response [IMP]
- regulation of striated muscle tissue development [IDA]
- regulation of transcription from RNA polymerase II promoter [ISO]
- regulation of transforming growth factor beta receptor signaling pathway [IDA, ISO]
- regulation of transforming growth factor beta2 production [ISO]
- response to hypoxia [ISO]
- signal transduction involved in regulation of gene expression [ISO]
- skeletal system development [IGI]
- somitogenesis [IMP]
- thyroid gland development [IGI]
- transcription from RNA polymerase II promoter [ISO, TAS]
- transdifferentiation [ISO]
- transforming growth factor beta receptor signaling pathway [IDA, ISO]
- transport [ISO]
Gene Ontology Molecular Function- DNA binding [ISO]
- R-SMAD binding [ISO]
- RNA polymerase II activating transcription factor binding [ISO]
- RNA polymerase II core promoter proximal region sequence-specific DNA binding [ISO]
- RNA polymerase II core promoter sequence-specific DNA binding transcription factor activity [ISO]
- SMAD binding [ISO]
- bHLH transcription factor binding [ISO]
- beta-catenin binding [ISO]
- chromatin DNA binding [IDA]
- chromatin binding [IDA]
- co-SMAD binding [ISO]
- collagen binding [IPI]
- core promoter proximal region sequence-specific DNA binding [ISO]
- double-stranded DNA binding [IDA]
- enzyme binding [ISO]
- identical protein binding [ISO]
- phosphatase binding [ISO]
- protein binding [IPI]
- protein binding transcription factor activity [ISO]
- protein heterodimerization activity [IDA]
- protein homodimerization activity [IDA, ISO]
- protein kinase binding [ISO]
- sequence-specific DNA binding [ISO]
- sequence-specific DNA binding transcription factor activity [IDA, ISO]
- transcription factor binding [IPI, ISO]
- transcription regulatory region DNA binding [ISO]
- transforming growth factor beta receptor binding [ISO]
- transforming growth factor beta receptor, pathway-specific cytoplasmic mediator activity [ISO]
- ubiquitin binding [ISO]
- ubiquitin protein ligase binding [ISO]
- zinc ion binding [ISO]
- DNA binding [ISO]
- R-SMAD binding [ISO]
- RNA polymerase II activating transcription factor binding [ISO]
- RNA polymerase II core promoter proximal region sequence-specific DNA binding [ISO]
- RNA polymerase II core promoter sequence-specific DNA binding transcription factor activity [ISO]
- SMAD binding [ISO]
- bHLH transcription factor binding [ISO]
- beta-catenin binding [ISO]
- chromatin DNA binding [IDA]
- chromatin binding [IDA]
- co-SMAD binding [ISO]
- collagen binding [IPI]
- core promoter proximal region sequence-specific DNA binding [ISO]
- double-stranded DNA binding [IDA]
- enzyme binding [ISO]
- identical protein binding [ISO]
- phosphatase binding [ISO]
- protein binding [IPI]
- protein binding transcription factor activity [ISO]
- protein heterodimerization activity [IDA]
- protein homodimerization activity [IDA, ISO]
- protein kinase binding [ISO]
- sequence-specific DNA binding [ISO]
- sequence-specific DNA binding transcription factor activity [IDA, ISO]
- transcription factor binding [IPI, ISO]
- transcription regulatory region DNA binding [ISO]
- transforming growth factor beta receptor binding [ISO]
- transforming growth factor beta receptor, pathway-specific cytoplasmic mediator activity [ISO]
- ubiquitin binding [ISO]
- ubiquitin protein ligase binding [ISO]
- zinc ion binding [ISO]
Gene Ontology Cellular Component
Mus musculus
PREY
CYLD
2010013M14Rik, 2900009M21Rik, C130039D01Rik, CDMT, CYLD1, EAC, mKIAA0849
cylindromatosis (turban tumor syndrome)
GO Process (13)
GO Function (6)
GO Component (7)
Gene Ontology Biological Process
- necroptotic process [IGI]
- negative regulation of NF-kappaB import into nucleus [ISO]
- negative regulation of NF-kappaB transcription factor activity [IBA, ISO]
- negative regulation of T cell differentiation [IDA]
- negative regulation of canonical Wnt signaling pathway [ISO]
- positive regulation of extrinsic apoptotic signaling pathway [IBA, ISO]
- protein K63-linked deubiquitination [IBA, ISO]
- protein deubiquitination [IMP]
- proteolysis [IBA]
- regulation of intrinsic apoptotic signaling pathway [IBA, ISO]
- regulation of microtubule cytoskeleton organization [ISO]
- regulation of mitotic cell cycle [IBA, ISO]
- ripoptosome assembly involved in necroptotic process [IMP]
Gene Ontology Molecular Function
Gene Ontology Cellular Component
Mus musculus
Affinity Capture-Western
An interaction is inferred when a bait protein is affinity captured from cell extracts by either polyclonal antibody or epitope tag and the associated interaction partner identified by Western blot with a specific polyclonal antibody or second epitope tag. This category is also used if an interacting protein is visualized directly by dye stain or radioactivity. Note that this differs from any co-purification experiment involving affinity capture in that the co-purification experiment involves at least one extra purification step to get rid of potential contaminating proteins.
Publication
Alternative Splice Forms of CYLD Mediate Ubiquitination of SMAD7 to Prevent TGFB Signaling and Promote Colitis.
The CYLD lysine 63 deubiquitinase gene (CYLD) encodes tumor suppressor protein that is mutated in familial cylindromatosus, and variants have been associated with Crohn disease (CD). Splice forms of CYLD that lack exons 7 and 8 regulate transcription factors and functions of immune cells. We examined the expression of splice forms of CYLD in colon tissues from patients with CD ... [more]
Gastroenterology Dec. 01, 2018; 156(3);692-707.e7 [Pubmed: 30315770]
Throughput
- Low Throughput
Curated By
- BioGRID