HSPA5
Gene Ontology Biological Process
- ER overload response [ISO]
- activation of signaling protein activity involved in unfolded protein response [ISO]
- cellular response to antibiotic [IEP]
- cellular response to glucose starvation [ISO]
- cellular response to interleukin-4 [ISO]
- cerebellar Purkinje cell layer development [ISO]
- cerebellum structural organization [ISO]
- maintenance of protein localization in endoplasmic reticulum [ISO, ISS]
- negative regulation of apoptotic process [ISO]
- negative regulation of transforming growth factor beta receptor signaling pathway [ISO]
- positive regulation of cell migration [ISO, ISS]
- positive regulation of protein ubiquitination [ISO]
- proteolysis involved in cellular protein catabolic process [ISO]
- response to endoplasmic reticulum stress [IDA]
- substantia nigra development [ISO]
- toxin transport [ISO]
Gene Ontology Molecular Function
Gene Ontology Cellular Component
- COP9 signalosome [ISO]
- cell surface [ISO]
- endoplasmic reticulum [IDA, ISO, TAS]
- endoplasmic reticulum chaperone complex [ISO]
- endoplasmic reticulum lumen [ISO]
- endoplasmic reticulum membrane [ISO]
- endoplasmic reticulum-Golgi intermediate compartment [ISO]
- extracellular vesicular exosome [ISO]
- focal adhesion [ISO]
- integral component of endoplasmic reticulum membrane [ISO]
- membrane [IDA, ISO]
- midbody [ISO]
- mitochondrion [IDA]
- myelin sheath [ISO]
- nucleus [ISO]
- plasma membrane [ISO]
- smooth endoplasmic reticulum [IDA]
EIF2AK3
Gene Ontology Biological Process
- ER overload response [ISO, ISS]
- SREBP signaling pathway [ISO]
- activation of cysteine-type endopeptidase activity involved in apoptotic process [ISO]
- angiogenesis [ISO]
- bone mineralization [ISO]
- calcium-mediated signaling [ISO]
- cellular response to glucose starvation [ISO]
- chondrocyte development [ISO]
- endocrine pancreas development [ISO, ISS]
- endoplasmic reticulum organization [ISO]
- endoplasmic reticulum unfolded protein response [ISO, ISS]
- fat cell differentiation [ISO]
- insulin-like growth factor receptor signaling pathway [ISO]
- intrinsic apoptotic signaling pathway in response to endoplasmic reticulum stress [ISO]
- lactation [ISO]
- negative regulation of apoptotic process [ISO]
- negative regulation of gene expression [ISO]
- negative regulation of myelination [ISO]
- negative regulation of translation [ISO]
- negative regulation of translation in response to stress [IDA]
- negative regulation of translational initiation in response to stress [TAS]
- ossification [ISO, ISS]
- pancreas development [ISO]
- positive regulation of endoplasmic reticulum stress-induced intrinsic apoptotic signaling pathway [ISO]
- positive regulation of gene expression [ISO]
- positive regulation of protein binding [ISO]
- positive regulation of signal transduction [ISO]
- positive regulation of transcription from RNA polymerase I promoter [ISO]
- positive regulation vascular endothelial growth factor production [ISO]
- protein autophosphorylation [IDA, IMP, ISO, ISS]
- protein homooligomerization [ISO, ISS]
- protein phosphorylation [IDA, IMP, ISO]
- regulation of fatty acid metabolic process [ISO]
- response to endoplasmic reticulum stress [ISO]
- skeletal system development [ISO]
- translation [ISO]
Gene Ontology Molecular Function
Gene Ontology Cellular Component
Affinity Capture-Western
An interaction is inferred when a bait protein is affinity captured from cell extracts by either polyclonal antibody or epitope tag and the associated interaction partner identified by Western blot with a specific polyclonal antibody or second epitope tag. This category is also used if an interacting protein is visualized directly by dye stain or radioactivity. Note that this differs from any co-purification experiment involving affinity capture in that the co-purification experiment involves at least one extra purification step to get rid of potential contaminating proteins.
Publication
Inhibition of glioma growth by minocycline is mediated through endoplasmic reticulum stress-induced apoptosis and autophagic cell death.
We have reported that minocycline (Mino) induced autophagic death in glioma cells. In the present study, we characterize the upstream regulators that control autophagy and switch cell death from autophagic to apoptotic.Western blotting and immunofluorescence were used to detect the expressions of eukaryotic translation initiation factor 2? (eIF2?), transcription factor GADD153 (CHOP), and glucose-regulated protein 78 (GRP78). Short hairpin (sh)RNA ... [more]
Throughput
- Low Throughput
Curated By
- BioGRID